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The elution order does not match the expected order for coconut oil and the peaks do not match. Explain what might cause this issue.
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- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.A dosage of 3 mcg/kg/min has been ordered for an 87.4 kg adult. The solution has a strength of 50 mg in 250 mL. Calculate the dosage per min.
- give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)
- A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?A dosage of 3 mcg/kg/min has been ordered for an 87.4 kg adult. The solution has a strength of 50 mg in 250 mL. Calculate the flow rate in mL/hr.
- fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.Consider the titration of 50.00 mL of 0.160 M NH3 with 0.200 M HCl. Calculate the pH at the following volumes of the titrant: VT = 0.00 (Initial Stage) VT = 20.00 VT = 30.00 VT = 40.00 (Equivalence Point Stage) VT = 45.00 Kb = 1.76 x 10-5If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?