The concentration of caffeine in an energy soft drink was determined by standard addition using HPLC. The following procedure was used: a standard caffeine solution was prepared by adding 0.09850g of caffeine to 25.0 cm3 of water.A series of solutions were prepared by adding known volumes of the standard solution to the soft drink before diluting to 100 cm3. The volume of standard and the caffeine peak area are shown in the table: Energy Drink (cm3) Added caffeine Std (cm3) Peak area 15 0 965 15 5 1352 15 10 1739 15 15 2126 15 20 25
The concentration of caffeine in an energy soft drink was determined by standard addition using HPLC. The following procedure was used: a standard caffeine solution was prepared by adding 0.09850g of caffeine to 25.0 cm3 of water.A series of solutions were prepared by adding known volumes of the standard solution to the soft drink before diluting to 100 cm3. The volume of standard and the caffeine peak area are shown in the table: Energy Drink (cm3) Added caffeine Std (cm3) Peak area 15 0 965 15 5 1352 15 10 1739 15 15 2126 15 20 25
Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter9: Column Chromatography: Fluorenone, Cholesteryl Acetate, Acetylferrocene, And Plant Pigments
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6.
The concentration of caffeine in an energy soft drink was determined by standard addition using HPLC. The following procedure was used: a standard caffeine solution was prepared by adding 0.09850g of caffeine to 25.0 cm3 of water.A series of solutions were prepared by adding known volumes of the standard solution to the soft drink before diluting to 100 cm3. The volume of standard and the caffeine peak area are shown in the table:
Energy Drink (cm3) |
Added caffeine Std (cm3) |
Peak area |
|
15 |
0 |
965 |
|
15 |
5 |
1352 |
|
15 |
10 |
1739 |
|
15 |
15 |
2126 |
|
15 |
20 |
2513 |
Determine the concentration of caffeine in the soft drink as mg cm-3.
- A.
3.3
- B.
2.3
- C.
4.3
- D.
7.3
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