The best combination of regeants for a typical PCR reacion would include the following concentration of reagents:   a.  1x buffer, 1.5 mMm MgCl2, 0.5nM primers, 10mMdNTPs, 2units Taq, 1010 copies of template b. none of these answers c. 1x buffer, 1.5 mMm MgCl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 105 copies of template d. 1x buffer, 1.5 mMm NH3 Cl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 102 copies of template e. 1x buffer, 1.5 mMm KCL2 , 0.5nM primers, 10mMdNTPs, 2units Taq, 103copies of template

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The best combination of regeants for a typical PCR reacion would include the following concentration of reagents:

 

a.  1x buffer, 1.5 mMm MgCl2, 0.5nM primers, 10mMdNTPs, 2units Taq, 1010 copies of template

b. none of these answers

c. 1x buffer, 1.5 mMm MgCl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 10copies of template

d. 1x buffer, 1.5 mMm NH3 Cl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 10copies of template

e. 1x buffer, 1.5 mMm KCL2 , 0.5nM primers, 10mMdNTPs, 2units Taq, 103copies of template

 

thank you!!

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