The absorption cross section for fluorescein, a dye that is used as a fluorescent probe for biological imaging (see the Chapter Opener image), is 9.25 × 10-16 cm2 molecule-1 at 500 nm. How much light is transmitted through a 1-cm path length by a standard 10-6 M solution of fluoresceinused to label biological samples?
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The absorption cross section for fluorescein, a dye that is used as a fluorescent probe for biological imaging (see the Chapter Opener image), is 9.25 × 10-16 cm2 molecule-1 at 500 nm. How much light is transmitted through a 1-cm path length by a standard 10-6 M solution of fluorescein
used to label biological samples?
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