Suppose you prepared one additional tube of albumin unknown and got an absorbance value of 1.99. What may you conclude from this tube and what is its significance to the experiment.
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- 1. Diuril is a prescription drug used to treat hypertension. You are given a 2.5 g vial of Diuril, with negligible volume, and asked to prepare a solution by combining with 20.0 mL of sterile water. What is the final concentration in mg/mL? 2. You are asked to prepare a bolus IV injection of a new, experimental MRI contrast agent. The reconstituted stock solution of the contrast agent has a concentration of 0.60 M. What volume of the stock solution do you need in order to prepare 5.0 mL of 0.20 M contrast agent? 3. Which of the following is not affected by the process of dilution? a. The Amount of Solvent b. Concentration c. The Amount of Solute d. Volume 4. A doctor orders 5.0 mg of Compazine, used to treat nausea and migraines. If the stock solution on hand is 2.5% (m/v), how many milliliters are administered to the patient? 5. Determine the molarity (M) of a solution prepared by dissolving 11.3 g NaOH to form 251.0 mL solution. 6. For the following reaction, how many mL of 0.200 M…4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.Question 1: Suppose you have given 5 tablets weighing 449mg, 450mg, 448mg, 451mg and 453mg, respectively. Calculate 300 mg equivalent sample weight for assay. The product claim is 500 mg Vitamin C is present in each tablet. Question 2: Volume of the standard sodium edetate is 12.6 mL (calculated after the assay of Zinc Sulfate Syrup). Determine the % of potency of the drug considering the claimed dose is 11.5You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
- 8. R ACULAR (ketorolac tromethamine) Ophthalmic Solution 0.5% Disp. 5 mL Sig: One drop q.i.d. prn allergic conjunctivitis How many milligrams of the active con- stituent would be present in each drop of the ophthalmic solution if the dropper service delivers 20 drops per milliliter? (a) 0.25 mg (b) 25 mg (c) 0.025 mg (d) 1.25 mg 8. (a) 0.25 mg ketorolac tromethamine8 You have a stock dye solution with a concentration of 0.25 mg/mi. You take 20 ul of this dve and place You nave ses tube filled with 40 ml of water (final volume). What is the amount of dye present in the glass tube?From the basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of light the beam transmitted through a 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 25mg/mL. Compare between 1 and 2.
- After performing the manual Albumin assay, you get the absorbance value of 0.205 for a 4.5 g/dL albumin standard and 0.114 for control A. What is the calculated value of the control? (just write a number with one decimal, the units will be mg/dL)34. A dermatologic lotion contains 1.25 mL of liquefied phenol in 500 mL. Calculate the percentage (v/v) of liquefied phenol in the lotion. 34. 0.25% v/v liquefied phenol1. The concentration of the glucose stock solution in bottle is 50 µM. This stock solution was diluted by a, factor of 10. If you took 25 ml out of the diluted solution and added it to a new test tube containing the following reagents 5 ml of water, 10 ml of cofactor and 10 ml of buffer. What is the final concentration of glucose in the new test tube?