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Should antibodies tests be used to diagnose a COVID 19 infection? In what situations are antibody tests useful?
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- What is the purpose of anti-human IgG antibodies in immunological testing?Why monoclonal antibodies method is better than polyclonal antibodies method in detecting specific pathogen? Explain the reasons for both selection or rejection methods..Which of the following would develop as a result of exposure to antigens in a COVID-19 vaccine?
- Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?All of the following are true concerning the Alere HIV test except: a)this test can detect infection 10 days post exposure b)the presence of three lines on the test indicates a positive reaction for anitgen and antibody against HIV c)unlike Oraquick the test is run with blood sample only and not oral fluid d)the presence of a single line indicates that a person has antibodies but no antigen for HIV e)this is a test that is considered both a direct and indirect test for HIV diagnosisHow reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..
- 1)Describe the key processes involved in vaccine manufacturing.2) Review the literature and identify the key players in the various mutants of the spikeprotein.3) How were the structures of ACE 2 and spike protein solved?4) Why is it easier to target a single mutant versus multiple mutants in the virus?5) There are several coronaviruses in the database, why is the SARS-CoV-2 sodangerous?6) Summarize the key difference in the invasion of cells by:a. HIVb. Adenovirusc. Anthraxd. SARS-CoV-2e. MERSWhat is the point to labeling a secondary antibody with a marker that can be visualized instead of just labeling the primary antibody?1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8
- A COVID-19 test, which produce result in 10 minutes, has the following test characteristics. The test has a 95% sensitivity and a 95% specificity. The State of Massachusetts is in dilemma whether to test symptomatic individuals or the entire residents to decrease the spread of infection as depicted in the following two scenarios. Scenario A. The State of Massachusetts wants to administer the test on 40,000 individuals who will develop cough, fever and shortness of breath in the next 10 days to identify COVID-19 infected individuals and institute public health measures. The estimated prevalence of COVID-19 in this population is 750 per 1,000 population using a gold-standard test. Scenario B. The State of Massachusetts wants to administer the test to its entire COVID-19 uninfected population (6,750,000). The estimated prevalence of COVID-19 in this population is 5 per 1,000 population using a gold-standard test. Please help the State of Massachusetts by responding to the…What are the important differences among a recombinant liveattenuated vaccine, a vector vaccine, and a subunit vaccine?If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidine