Select all examples of DNA transformation Check All That Apply Transfer of DNA through a pilus into a bacterial cell in a laboratory. Transfer of DNA from media into a bacterial cell in a laboratory. Transfer of DNA through a pilus into a bacterial cell in seawater. Transfer of DNA from the environment into a bacterial cell via a surface receptor.

Select all examples of DNA transformation Check All That Apply Transfer of DNA through a pilus into a bacterial cell in a laboratory. Transfer of DNA from media into a bacterial cell in a laboratory. Transfer of DNA through a pilus into a bacterial cell in seawater. Transfer of DNA from the environment into a bacterial cell via a surface receptor.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
When an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugation
In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. indicate which plasmids were successfully digested by the endonuclease. act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.
Describe the mechanisms of horizontal gene transfer in bacteria, describing the manner in which the DNA can be transferred from one cell to another.Choose a disease or an organism that has a well-documented mechanism of resistance (such as methicillin resistant Staphylococcus aureus - MRSA or tuberculosis) and identify the gene or genes that confer resistance and the method of DNA transfer thought to contribute to its spread. This may be from one organism to another or may simply be passed down from mother to daughter cell due to environmental pressure.
Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonuclease
SC DF eas fill The diagram below illustrates how recombinant DNA technology is used to produce valuable products. What is the purpose of using restriction enzymes in recombinant DNA technology? PDF UNOFFIC TRANSCR ostly cloudy DNA plasmids OO Bacterial cell (E. coli) Bacterium with recombinant DNA plasmid Plasmids isolated OO DO Cloned cells produce new protein such as enzymes or hormones O-C Ligase joins sticky ends Replication of new gene 00 00 Restriction enzyme cuts plasmids Sticky ends Gene obtained from donor DNA using the same restriction enzyme O The restriction enzymes are used to catalyze the synthesis of new proteins such as enzymes or hormones. Sticky ends O The restriction enzymes are used to join sticky ends of both the cut plasmid and the cut gene obtained from donor DNA PI mas omis O Search
.Describe the method you would use to test for the uptake of a plasmid with a DNA insert
Sequence the steps to transfect and observe a fluorescent protein in a slice of the brain: * Place the slice of brain under the microscope and observe it. * Wait for the expression of the fluorescent protein by the transfected cells. * Harvest and culture a slice of brain. * Electropore in the slice a plasmid coding for a fluorescent protein.
In your own words, explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the expression of the gene could be monitored or selected for in the lab, and how the protein coded from this DNA sequence could potentially be purified. You must include at least 6 of the following terms in your answer: transformation, plasmid, antibiotic selection, fusion protein, chromatography, reporter gene, bioluminescence, competent cells, electroporation, calcium chloride.
Define the following terms: Genome Bacteriophage λ DNA Plasmid Restriction Enzyme Standard Curve Plasmid Map
Which of the following single-stranded DNA molecules may possibly be a restriction enzyme target cut site in the double-stranded state? O ATGCCGTA GTATCTAT OATGCTACC OGTCATGAC
Diagram the steps for the production of recombinant DNA. Label and Identify the steps involved and the protein/enzyme requirement for each.