Ribosoms were isolated from bacteria grown in a "heavy" medium (13C and 15N) and from bacteria grown in "light" medium (12C and 14N). These 70S ribosoms were added to an in vitro system engaged in protein synthesis. An aliquot removal serveral hours later was analyzed by density-gradient centrifugation. How many bands of 70S ribosoms would you expect to see in the density gradient?
Follow up question:...
The answer given in problem 31. 17 is inadequate for me, and I simply don't understand the explanation for the answer...
The problem is: Ribosoms were isolated from bacteria grown in a "heavy" medium (13C and 15N) and from bacteria grown in "light" medium (12C and 14N). These 70S ribosoms were added to an in vitro system engaged in protein synthesis. An aliquot removal serveral hours later was analyzed by density-gradient centrifugation. How many bands of 70S ribosoms would you expect to see in the density gradient?
Is it possible to give a more comprehensive explanation? :)
Introduction :-
A ribosome is a sophisticated molecular machine that produces proteins from amino acids during the process of protein synthesis, also known as translation. Protein synthesis is a fundamental operation that all live cells must carry out.
Answer :-
One light band, one heavy band, a hybrid of light 30S and heavy 50S, and a hybrid of heavy 30S and light 50S are among the four bands expected.
Follow-up question
Why is the bands mentioned to be expected? :)
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