R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp
R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter17: Metabolism: An Overview
Section: Chapter Questions
Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...
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![Amp
8,000
Xhol
0 (or 12,000)
I
PUC 18
12,000 bp
2,000
insert
4,000
EcoRI alone
HindIll alone
Xhol alone
EcoRI + Hindlll
EcoRI + Xhol
HindIll+ Xhol
12,000 bp
12,000 bp
12,000 bp
3,000 and 9,000 bp
2,000 and 10,000 bp
5,000 and 7,000 bp
You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part)
contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction
enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted
DNA fragment is indicated by the striped bar. You perform several restriction digests of the
recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of
the resulting DNA fragments are shown in the table
+
30) At what position number on the plasmid is the HindIll site?
a) 2,000 bp
b) 3,000 bp
c) 6,000 bp
d) 8,000 bp](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcd333dae-61d9-4d50-8705-d3033b401c01%2Fe4363369-1602-4d0f-a3a4-c3d6e0cd2258%2F5e6y9h_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Amp
8,000
Xhol
0 (or 12,000)
I
PUC 18
12,000 bp
2,000
insert
4,000
EcoRI alone
HindIll alone
Xhol alone
EcoRI + Hindlll
EcoRI + Xhol
HindIll+ Xhol
12,000 bp
12,000 bp
12,000 bp
3,000 and 9,000 bp
2,000 and 10,000 bp
5,000 and 7,000 bp
You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part)
contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction
enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted
DNA fragment is indicated by the striped bar. You perform several restriction digests of the
recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of
the resulting DNA fragments are shown in the table
+
30) At what position number on the plasmid is the HindIll site?
a) 2,000 bp
b) 3,000 bp
c) 6,000 bp
d) 8,000 bp
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