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- Question 33 Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? ddATP ddGTP ddCTP ddTTP A) GGTAACC в) ССААТGG GGTTACC D) CCATTGG | | | |Question #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…Question 43 The addition of restriction endonucleases in the cloning process is done following the ligation with DNA ligase. A) True B) False
- Question 33 RNA polymerase IlI: A is located in the nucleoplasm and transcribes the protein-encoding genes through mRNAs. B transcribes tRNA genes and protein transport genes. c) transcribes the 55 RNA genes. D) transcribes RNA genes associated with TRNA processing.Question 19 A transversion mutation would be replacing T by: A either A or G BU (c) T D) cQuestion 46 Cellular transcription is : O Use of DNA as a template to make complementary DNA Use of DNA as a template to make complementary RNA O Use of RNA as a template to make proteins
- Question 5 Both restriction enzymes and DNA ligases can form phosphodiester bonds. (A) True B) FalseQuestion 18 Match Column A with Column B. transports amino acids to the site of protein synthesis A. operator B. rRNA has anticodon loop C. IRNA v directs amino acid sequence of proteins D. MRNA contains the Shine-Delgarno sequence E. most type of RNA v synthesized in the eukaryote nucleus F. promoter v found inside the ribosome v region where the RNA binds to the DNA template region where the activator protein binds to the DNA templateQuestion 11 The triplet code allows many amino acids to be specified by more than one codon. Such a code is said to be A synonymous. B) degenerate. c unambigous D) universal.
- Question 5 Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. A) True B) FalseQuestion 6 The helical turns of the DNA does not only provide spaces for binding with regulatory proteins but they cause the molecule to resemble a spiral staircase. A) True B) FalseQuestion 7 A unique 5'CCA terminal sequence is found in all RNAS to be able to carry the correct amino acid to the ribosome. A True B) False