Answered: Plot the appropriate graphs to find: -…

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Plot the appropriate graphs to find: - The approximate Vmax from the Michaelis-Menten curve.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.5: Blood Glucose And Hemoglobin A1c

Problem 11RQ

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Question

The SARS-Cov-2 virus is the virus that cause COVID-19. The viral protease is a known target for finding potential viral inhibitor. The activity of the viral protease can be assayed using the synthetic peptide substrate attached to 7-amino-4-carbamoylmethylcoumarin (PACC). The viral protease cleaved the substrate producing the fluorogenic ACC which can be monitored using a spectrofluorometer. The following is data from SARS-Cov-2 protease (0.1 mg of enzyme) upon reaction with various concentrations of the substrate PACC.

Plot the appropriate graphs to find:

- The approximate Vmax from the Michaelis-Menten curve.

Time
(min)
1
5
10
20
40
60
90
120
1 μM
PACC
substrate
0.03
0.03
0.03
0.07
0.15
0.23
0.37
0.41
2 μM
PACC
substrate
0.06
0.06
0.06
0.09
0.27
0.43
0.67
0.85
ACC (janole)
3 μM
4 μM
6 μM
20 μM
PACC
PACC
PACC
PACC
substrate substrate substrate substrate
0.09
0.09
0.09
0.13
0.35
0.56
0.99
1.26
0.12
0.12
0.12
0.17
0.47
0.79
1.29
1.72
0.18
0.18
0.18
0.26
0.64
1.05
1.87
2.21
0.28
0.28
0.28
0.36
1
1.65
2.57
3.14
40 μM
PACC
substrate
0.39
0.39
0.39
0.47
1.17
1.91
3.20
3.52

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From the graphs given, can you find the K_mfrom the Michaelis-Menten curve and the Lineweaver-Burke plot.

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