ChemistryPlease estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3 2. AldolaseAldolase is a 157 kD enzyme that catalyzes the following reaction:AldolaseOHHOOHD-Fructose-1,6-diphosphate2.551025501[s] (uM) Vo (uM/min)13.98.115.322.028.033.0rofalanDihydroxyacetone phosphateD-Glyceraldehyde-3-phosphateIn an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detectedby modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). Theenzyme concentration for the assay is 150 pM, and the following data is obtained:OHH₂NNH₂OH

Estimation of Km and Vmax can be done from the Michaelis-Menten equation. The values of Km and Vmax…

Answered: Please estimate the Km and Vmax and…

Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3

Chemistry & Chemical Reactivity

10th Edition

ISBN:9781337399074

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions

Section14.7: Reaction Mechanisms

Problem 1.1ACP

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