One way to prepare the HEPES buffer involves preparing solutions of the sodium salt form of HEPES (A) and the weak acid form of HEPES (HA) and then combining the solutions to obtain a buffer with the desired pH and concentration. CORRECT; see section 2.4 In preparing 250 mL of 1.0M stock solutions of the A and HA forms of HEPES (supplied as solids by the manufacturer with molar masses of 260.3g.mol-¹ and 238.3g.mol-¹, respectively), what masses of the acid (HA) and the conjugate base (A) would be needed? Round to the nearest whole number. mass of HA (g) = 59.58 mass of A (g) = 65.08 (Tolerance is +/- 2%) eTextbook and Media Hint X INCORRECT; see section 2.4 Calculate the volume of each stock solution needed to prepare 1.0 L of the 0.10 M HEPES buffer at a pH = 8.0. volume HA (mL) = i 100 volume A (mL) = i 100 (Tolerance is +/- 2%)
One way to prepare the HEPES buffer involves preparing solutions of the sodium salt form of HEPES (A) and the weak acid form of HEPES (HA) and then combining the solutions to obtain a buffer with the desired pH and concentration. CORRECT; see section 2.4 In preparing 250 mL of 1.0M stock solutions of the A and HA forms of HEPES (supplied as solids by the manufacturer with molar masses of 260.3g.mol-¹ and 238.3g.mol-¹, respectively), what masses of the acid (HA) and the conjugate base (A) would be needed? Round to the nearest whole number. mass of HA (g) = 59.58 mass of A (g) = 65.08 (Tolerance is +/- 2%) eTextbook and Media Hint X INCORRECT; see section 2.4 Calculate the volume of each stock solution needed to prepare 1.0 L of the 0.10 M HEPES buffer at a pH = 8.0. volume HA (mL) = i 100 volume A (mL) = i 100 (Tolerance is +/- 2%)
General Chemistry - Standalone book (MindTap Course List)
11th Edition
ISBN:9781305580343
Author:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Chapter16: Acid-base Equilibria
Section: Chapter Questions
Problem 16.147QP
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Question
Transcribed Image Text:One way to prepare the HEPES buffer involves preparing solutions of the sodium salt form of HEPES (A) and the weak acid form of
HEPES (HA) and then combining the solutions to obtain a buffer with the desired pH and concentration.
CORRECT; see section 2.4
In preparing 250 mL of 1.0M stock solutions of the A and HA forms of HEPES (supplied as solids by the manufacturer with molar
masses of 260.3g.mol-¹ and 238.3g.mol-¹, respectively), what masses of the acid (HA) and the conjugate base (A) would be
needed? Round to the nearest whole number.
mass of HA (g) = 59.58
mass of A* (g) = 65.08
(Tolerance is +/- 2%)
eTextbook and Media
Hint
X INCORRECT; see section 2.4
Calculate the volume of each stock solution needed to prepare 1.0 L of the 0.10 M HEPES buffer at a pH = 8.0.
volume HA (mL) = i 100
volume A (mL) = i
(Tolerance is +/- 2%)
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