One of the components in TAE is 0.5 M EDTA. Answer the following: a. How much EDTA is needed to prepare 100 ml of 0.5 M EDTA? EDTA MW = 372.2 g/mole b. As EDTA dissolves in water, the [H+] will increase (note that full name of EDTA includes "acetate"). To achieve a pH = 8.0, would you need to add HCl or NaOH? c) When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose is needed to make this solution?

One of the components in TAE is 0.5 M EDTA. Answer the following: a. How much EDTA is needed to prepare 100 ml of 0.5 M EDTA? EDTA MW = 372.2 g/mole b. As EDTA dissolves in water, the [H+] will increase (note that full name of EDTA includes "acetate"). To achieve a pH = 8.0, would you need to add HCl or NaOH? c) When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose is needed to make this solution?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter17: Complexation And Precipitation Reactions And Titrations

Section: Chapter Questions

Problem 17.3QAP

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