One microgram of your LDH catalyzed NAD* reaction at a rate of 0.50 µmole/min under optimum conditions. LDH molecular weight is 140,000 da. (a) What is the specific activity of your LDH? (b) What is kcat, the turnover number.

One microgram of your LDH catalyzed NAD* reaction at a rate of 0.50 µmole/min under optimum conditions. LDH molecular weight is 140,000 da. (a) What is the specific activity of your LDH? (b) What is kcat, the turnover number.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter31: Immunochemistry

Section: Chapter Questions

Problem 31.75P

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Vitamins and Coenzymes

A class of organic compounds needed in the human body for normal activities is known as vitamins. They are characterized into two parts depending upon their solubility in water. They can act as coenzymes, cofactors, or prosthetic groups for enzymes.

Question

One microgram of your LDH catalyzed NAD* reaction at a rate of 0.50 µmole/min under
optimum conditions. LDH molecular weight is 140,000 da.
(a) What is the specific activity of your LDH?
(b) What is kcat, the turnover number.

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