Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml O 2.37 x 10^11 pfu/ml O 2.37 x 10^5 pfu/ml 23.7 x 10^8 pfu/ml
Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml O 2.37 x 10^11 pfu/ml O 2.37 x 10^5 pfu/ml 23.7 x 10^8 pfu/ml
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A.
From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix
100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C.
The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in
your Bacteriophage-T4 stock culture?
O 237 x 10^10 pfu/ml
2.37 x 10^8 pfu/ml
2.37 x 10^11 pfu/ml
2.37 x 10^5 pfu/ml
O 23.7 x 10^8 pfu/ml](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fccc4908a-ff27-4156-943f-bb76251740f8%2Fff216102-6637-4c5e-bd27-a8565054fe43%2Ftukoero_processed.png&w=3840&q=75)
Transcribed Image Text:Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A.
From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix
100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C.
The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in
your Bacteriophage-T4 stock culture?
O 237 x 10^10 pfu/ml
2.37 x 10^8 pfu/ml
2.37 x 10^11 pfu/ml
2.37 x 10^5 pfu/ml
O 23.7 x 10^8 pfu/ml
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