LAB QUESTIONS – ASSESSMENT AND CRITICALTHINKING1. When is a simple negative stain used?2. Why do microorganisms remain unstained in the simplenegative staining procedure?3. What information can be obtained from a simple positivestaining procedure?4. What is the difference between a simple and differentialstain?5. What is the reagent and purpose for each of the followinggram stain steps?a. Primary stainb. Mordantc. Decolorizerd. Counterstain6. Which step is the most crucial or most likely to cause poorresults in the Gram stain? Why?7. What part of the bacterial cell is most involved with Gramstaining, and why?8. What cell wall component is responsible for the acid-fastproperty of mycobacteria?9. Is a gram stain an adequate substitute for an acid-fast stain?Why or why not?10. Which area on a streak plate will contain the greatestamount of growth? The least amount of growth? Explainyour answers.11. What is a colony (as viewed on an agar plate)? How can apure culture be obtained from a mixture of bacteria?12. What is a selective agar? Why can it be of importance?13. What is a differential agar? Why can it be of importance?14. Describe how the following agars work: MacConkey,Blood, Phenylethyl alcohol, Mannitol salt.15. What are the IMVIC series of tests? WHY / HOW are theyused?16. What is the catalase test? How is it used for colonies thatare gram positive?17. What is sputum and why is it important formicrobiological testing?18. What is the reason for testing staphylococci with acoagulase test?19. What is the importance of group B Streptococcusagalactiae?20. Starting with the MacConkey agar and IMVIC tests.Describe how a bacterium can be biochemically identifiedas Escherichia coli.

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LAB QUESTIONS – ASSESSMENT AND CRITICAL THINKING 1. When is a simple negative stain used?

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