in HPLC, what does it mean to equilibrate the system. Let us assume that the system is currently running 100%B. You have told them to run the first standard of caffeine using Reverse Phase chromatography with a C18 column, 30% A (Water/0.1%TFA) and 70% B (MeOH/0.1%TFA). What should I equilibrate the system to and for how long?
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in HPLC, what does it mean to equilibrate the system.
Let us assume that the system is currently running 100%B. You have told them to run the first standard of caffeine using Reverse Phase chromatography with a C18 column, 30% A (Water/0.1%TFA) and 70% B (MeOH/0.1%TFA).
What should I equilibrate the system to and for how long?
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Solved in 2 steps
- 16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC2Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (i) (ii) (iii) (iv) 0.01 TO 0.8 1.2 0". BEO - 2.0 1757 $122. 2.8 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?3. This is the standard procedure for determination of nicotine in urine. A 1.00mL sample was placed in a 12mL vial 5-aminoquinoline was added as the internal standard (5 ug). The sample was monitored using mass spectrometry at m/z values for nicotine (84) and the internal standard (144). Given the following data determine the concentration of the two unknown samples Nicotine (ug/L) Area Area 12 0.056 0.059 51 0.402 0.391 102 0.684 0.669 157 1.011 205 Unknown 1 Unknown 2 1.278 0.51 1.18 1.063 1.355 0.53 1.32
- Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.You are given the suggestion that a 0.15 ppm quinine aqueous solution is a good concentration to shoot for in the experiments, but quinine fluoresces best when it is acidified to 0.10 M H2SO4. Describe how to make 100 mL of the recommended solution starting with quinine sulfate (actually, quinine hemisulfate salt monohydrate). Assume you also have 1.0 M sulfuric acid and de-ionized water. Describe the process of a substance undergoing fluorescence and compare to the process of phosphorescence.You find that for your analysis, your response factor (adjusted for internal standard) for a particular analyte was 0.0104 ppm. When you run a sample mixture, the integration for that component is 2,186, and the integration for the internal standard is 14,139. What is the concentration of the component in ppm? (Enter the numerical value without units.)
- A chromatography column has a stationary phase volume that is 0.130 times the volume of the mobile phase. Find the retention factor, k, for a solute with each of the given distribution constants, Kp. Кр 3D 2.68 k = Kp = 19.1 k =You are given the suggestion that a 0.15 ppm quinine aqueous solution is a good concentration to shoot for in the experiments, but quinine fluoresces best when it is acidified to 0.10 M H2SO4. Describe how to make 100 mL of the recommended solution starting with quinine sulfate (actually, quinine hemisulfate salt monohydrate). Assume you also have 1.0 M sulfuric acid and de-ionized water.9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B (pyridoxine hydrochloride) are determined by capillary zone electrophoresis (CZE) using a pH 9 sodium tetraborate-sodium dihydrogen phosphate buffer or by micellar electrokinetic chromatography (MEKC) using the same buffer with the addition of sodium dodecyl sulphate. Detection is by UV absorption at 200 nm. CH₂OH pka 5.5 pka 9.7 ΝΗ -OH HOH₂C. N NH₂ pKa 3.6 NH₂ HO -OH Vitamin B3 (weakly basic) Vitamin B6 (weakly acidic) Vitamin B₁ (weakly basic) HO- CH₂OH Vitamin B₂ (weakly acidic) a The elution order when using MEKC is vitamin B3 (5.59 min), vitamin B6 (6.60 min), vitamin B₂ (8.82 min) and vitamin B₁ (11.22 min). Comment on the solubility of the B vitamins in the sodium dodecyl sulphate micelles? The sodium dodecyl sulphate micelles elute at 17.8 min. OH pka 9.0
- Injection of ethane, propane, butane, pentane, and hexane on a squalene column under isothermal conditions gives retention times of 3.51, 4.98, 7.30, 11.00, and 16.85, respectively. Air, which is nonretained, elutes at 1.00 min. An injection of pyridine under the same conditions gives a retention time of 10.05 min. What is the Kováts retention index for pyridine under these conditions? O A. 334 O B. 300 O C. 485 O D. 478 O E. 370On a 0.109-mm-diameter column with a 0.813-μm-thick stationary phase, an unretained solute passes through the column in 2.16 min, while the analyte requires 8.29 min. Calculate the phase ratio, β, for this column. what is β? what is K (partition coefficient)?A simple paper chromatography experiment involved the analysis of the food dyes in lollipop samples. A chocolate-flavored lollipop was dissolved using various solvents and was spotted in the chromatogram (E). Four food dye standards (blue, green, red, and orange) were tested alongside the chocolate-flavored lollipop. The mobile phase used for the analysis was 0.1% NaCl. After the analysis, the resulting chromatogram revealed the separation of three colors, which were attributed to the food dyes indigo carmine (blue), tartrazine (yellow), and erythrosine (red). 10 Ruler is in cm unit в с B C D A E A E BEFORE AFTER Calculate the rf for each dye. Express answers in 3SF. erythrosine: indigocarmine: tartrazine: Which dye is the most polar? Choose.. Which dye is the least polar? Choose...