In flow cytometric analysis of leukocytes, side scatter measurement is used to estimate which of the following:   Question 9 options:   A)  cell volume   B)  nuclear volume   C)  platelet volume   D)  cytoplasmic granularity

Question 1 An 18yrs old woman from Ananekrom in the Asante- Akyem North District was presented to your consulting room with the following complaints; headache, photophobia, painful and stiff neck as well as vomiting. Upon physical examination, the patient was febrile(temperature, 38°C) with tachycardia. Her CSF sample was taken to the laboratory for further investigation. Her results came out with low glucose estimation of 1.2mmol/L and high protein estimation of 10.0g/dL. Gram staining results show numerous Neutrophils with Gram positive diploccoci- +++ (A) What condition does this patient's laboratory and other Findings suggest?

What is hemocytometry, and which device is used for calculating cells using this technique?

Can all abnormal hemoglobins be diagnosed by electrophoresis? Explain your answer in detail.

Can all abnormal hemoglobin be diagnosed by electrophoresis? Explain your answer in detail.

Melinda was working the day shift in the hematology laboratory.  The laboratory’s protocol called for three levels of blood cell controls to be run at the following times: 1) at the beginning of the shift, 2) within each run of patient samples during the day and 3) any times reagents were changed.  The mean for the low (abnormal) control for the red blood cell count was given as 2.00 x 10^12/L, the standard deviation was 0.15, and the confidence limit (acceptable control range) was 2.00 x 10^12/L +/- 2sd (or +/- 0.3). The first morning low control result was 2.10 (x 10^12/L).  In five subsequent runs, the low control results were 2.16, 2.19, 2.20, 2.22 and 2.25. 1.  Do these values represent a shift, a trend, or neither? 2.  Should Melinda be concerned about these values?  Explain.   3.  Does Melinda need to take any action?

a) Hemoglobin accounts for 95% of the protein in red blood cells.  If you Google the number of hemoglobin molecules in a RBC, most sources state 250-270 million hemoglobin molecules.  If hemoglobin has a molecular weight of 67,000 Da, what is the concentration of hemoglobin in RBCs?  Show your work and show your units. Use dimensional analysis to have your final answer in moles per liter. B) The hemoglobin molecule is nearly spherical with a diameter of 55 Angstroms. You can look at the 3D structure of human hemoglobin on the RCSB PDB website.  Calculate the volume of a hemoglobin molecule, and can the number of hemoglobin molecules quoted in Question a actually fit into one red blood cell?

In one square of a hemacytometer you count 55 cells.  What is the approximate concentration of cells per l (liter) in this sample?  (note that "^" means superscript, e.g. 10^2 means 10 to the 2nd power) The options are 5.5 X 10^5 2.75 X 10^8 5.5 X 10^8 2.75 X 10^5

A White blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume). 7 square is counted and the values are; Total Viable Cells: 168 Total Nonviable Cells:32 a.Percentage of Viable Cells: ? b.Average Number Of cells per square: ? c.Dilution Factor:? d.Concentration (Viable Cells/ml): ?

1.)  A.) Define anemia: ? B.) Name the blood test that measures packed cell volume: ___?___  C.) The normal percentage of red blood cells for a human male is ____?____.   while it is ____?____ for a human female