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In determing Rh group, do you consider the control tube for reading when it shows an agglutination reaction? Kindly explain
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- The blood bank reported that an ether eluate showed an autopanagglutinin, and that an anti-e specificity was also detected. The patient’s Rh phenotype was D+, C+, E-, c+, e+, with a probable genotype of CDe/cde. A warm autoabsorption procedure revealed no underlying alloantibodies. He did not respond to steroids over the next month, and a splenectomy was performed. He was transfused a total of five units of e-negative red cells. His prednisone dosage was gradually decreased, and two months later he was maintaining a hematocrit of 40%. Assume that the patient had a routine CBC and differential performed 6 months after the hospitalization described above. List the pertinent RBC morphology finding(s) you would expect on the differential. Explain you answer. 8) What type of anemia did the patient experience during his hospital stay? Be specific. Explain the mechanism. 9) Give a logical explanation for the patient being started on prednisone. Please answer question 5 to 9 Your answerName the cells in the islets of langerhans which make glucagon and summarize the principles and procedures specific to how these cells were identified in immunostaining. word limit 250What is the recommended method to identify an antibody causing a suspected ABO based HDN( Hemolytic Disease of newborn)?
- Order: Xanax 0.5 mg po bid. Supply Xanax 0.25 mg tablets. How many tablets would you administer? Order: Penicillin 800,000 units po q4h x 10 days. Supply 1 tablet equals 400,000 units. How many tablets will you order? Order Zithromax: 400 mg po every day x 4 days. Supply: Zithromax 200 mg/5mL. What is the quantity to administer?RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…
- Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.Provide the correct susceptibility ratingA physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the medical technologist decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Using the IAT results, should the patient be considered Rh positive or Rh negative? Justify.
- A physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the physician decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Answer the following questions: Which conditions warrant the initial Anti-D result? Which specific test was done to confirm an initial Rh negative result? State the principle of the test, its clinical applications, and procedure.When will the antibody titer test most likely yield a positive result? On the day the symptom/s occurred 3 days after the onset of symptom/s O At least 7 days after the onset of symptom/s O 5 days after the onset of symptom/s O In the presymptomatic stageRewrite the following sentences after correction: 1. The N1303k mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. 2. If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates blood type B. 3. Indirect ELISA can detect polygenic gene expression.
