If after adding sulfanilic acid and naphthylamine to your nitrate broth cultures, your tube tube turn This means... the bacteria did not use nitrate (NO3). O the bacteria reduced nitrate to nitrite (NO₂). O you need to add zinc dust as the next step. the bacteria converted nitrate to ammonia (NH3).
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- Which of the following is TRUE about the Nitrate Reduction test? Question 1 options: A Nitrate oxidase is the enzyme present for a positive test result B Pseudomonas aerugenosa can use oxygen instead of NO2 for aerobic respiration C Ammonia is reduced to NO2 by some bacteria D Oxygen is needed to reduce nitrate E It tests the ability to reduce NO3 to NO2 or ammonia or nitrogenI have another one, 1 ml of sewage water is added to 9 ml of water. 0.1 ml of this is added to 9.9 ml of water. 1 ml of this is then added to 9 ml of water. 1 ml is plated and 243 colonies form. What is the CFU/ml of the sewage water? Thanks again.Young woman applies to her health center with burning and frequent vomiting but has no fever. The doctor diagnoses acute cystitis and the patient is allowed to leave a urine sample. a. How should the urine sample be taken? And why is this technology chosen? b. A rapid test is performed on the urine sample via a test strip that has reagent fields for nitrite. After 1 min read the strip which is pink colored. Which bacterial species is most likely in the urine sample? c. What is the reservoir for this urinary tract pathogen?
- What is the purpose of the vial that does not contain chara? (the solution with chara turned green showing that it is neutral) (the btb solution was yellow so it was acidic)a. This bacterium is a gram-negative cocci. It can ferment glucose, meaning it is positive for fermentation of glucose test. It can also reduce nitrate. What is it? b. This bacterium is a gram-positive rod. It is negative for the catalase reaction, but does produce gas from glucose. What is it?Bacteria should grow on plates with media containing . ..... to perform blue/white screening x gal kanamycin beta galatoside none All
- (c) Public health scientists wanted to investigate the effect of ionising radiation on the growth of Salmonella typhimurium in the presence of antibiotics. They grew bacterial colonies of Salmonella typhimurium in petri dishes labelled A, B and C. The Petri dishes were kept at 36°C for 48 hours, with scientists observing the bacteria every 24 hours. The results for this investigation are below. Petri dish A B с Treatment Was subjected to ionising radiation followed immediately by a dose of antibiotic X An equal amount of antibiotic X was added (as was added to Petri dish A) Was given neither ionising radiation nor antibiotic X After 24 hours Small spots of growth on surface No growth Growth across whole surface After 48 hours (ii) Identify the purpose of petri dish C. Large spots of growth on surface No growth Growth across whole surface (i) Explain the effect of the ionising radiation on the bacteria. (iii) Explain why scientists must continue to develop new antibiotics.The agar turns pink because the enzyme produces blank which causes a ph change to blank?Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test
- Which of the the following results of the extracellular enzyme tests is FALSE? Question 3 options: A Liquid gelatin positive for gelatinase B Clear area around bacterial growth on a starch plate after iodine is added means amylase is present C Clear zone around bacterial growth on a milk plate means caseinase is present D Solid gelatin after is positive for gelatinase E No clear zone around bacterial growth on milk plate means absence of casienasehow do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to sterilize the inoculating loop. The flame kills microorganisms that float in the air and could contminate samples. To sterilize the lid of the agar plate. To reduce the spread of antibiotic resistance.