If a mutation occurred that prevented single stranded binding proteins from binding, the result would be: O Replication would not be initiated O DNA would be synthesized on the leading strand, but not the lagging strand Replicating would be initiated, DNA would unwind, RNA primers would be laid down, but DNA polymerase would not bind The newly synthesized RNA would not be able to leave the DNA template Replication would be initiated, but RNA primers would not anneal to the DNA

If a mutation occurred that prevented single stranded binding proteins from binding, the result would be: O Replication would not be initiated O DNA would be synthesized on the leading strand, but not the lagging strand Replicating would be initiated, DNA would unwind, RNA primers would be laid down, but DNA polymerase would not bind The newly synthesized RNA would not be able to leave the DNA template Replication would be initiated, but RNA primers would not anneal to the DNA

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Description: If a mutation occurred that prevented single stranded binding proteins frombinding, the result would be:O Replication would not be initiatedO DNA would be synthesized on the leading strand, but not the lagging strandReplicating would be initiated, D

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 22P

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If a cell has trouble unwinding the DNA and relieving strain far ahead of the replication fork, in which of the following genes is there a mutation? Beve reverse transcriptase O gyrases/topoisomerases O single stranded binding proteins O ligase O helicase
During DNA replication, one of the new strands of DNA is synthesized continuously, while the other is synthesized as a number of separate fragments of DNA that are subsequently linked by DNA ligase. This is because O replication starts at many points on the chromosome RNA primers only anneal to one of the parental strands of DNA one of the parental strands is unwound slower than the other by helicase DNA polymerase III only synthesizes DNA in the 5' - 3' direction
DNA is made of two strands that are antiparallel. If one strand runs from 3’ to 5’ direction the other one will go from 5’ to 3’ direction. During replication or transcription, whatever the process is, it will always follow the 5’ to 3’ direction using the 3’ to 5’ directed strand as the template strand. Therefore, if following is the DNA sequence 5’-CCG ATC GCA CAA-3’ Using this sequence as template after transcription no protein can be translated. Why? Presence of start codon Absence of start codon Due to mutation If you want to start the translation, what change you need in the second codon (from 5’ to 3’ direction)? Substitution of C with G No change4 Deletion of Both I & III
Which of the following is not necessary for replication to proceed? O MRNA O RNA primer O DNA polymerase O primase O DNA ligase
DNA is made of two strands that are antiparallel. If one strand runs from 3’ to 5’ direction the other one will go from 5’ to 3’ direction. During replication or transcription, whatever the process is, it will always follow the 5’ to 3’ direction using the 3’ to 5’ directed strand as the template strand. Therefore, if following is the DNA sequence5’-CCG ATC GCA CAA-3’a) Using this sequence as template after transcription no protein can be translated. Why? I. Presence of start codonII. Absence of start codonIII. Due to mutationb) If you want to start the translation, what change you need in the second codon (from 5’ to 3’ direction)?I. Substitution of C with GII. No changeIII. Deletion of CIV. Both I & III
Considening the structure of DNA, what kind of bonds hold one nucleotide to another within one side of the DNA molecule? O hydrogen O phosophodiester O lonic peptide
During replication of a DNA molecule, the daughter strands are not produced in exactly the same manner. One strand, the leading strand, is made continuously toward the replication fork, while the lagging strand is made in fragments in the opposite direction. This difference in synthesis of the two strands is the result of which of the following? O a. DNA polymerase is not efficient enough to make two "good" strands of DNA O b. The two template strands are antiparallel, and DNA polymerase can only synthesize DNA in the 5' to 3' direction O c. The lagging strand is the result of DNA breaks caused by exposure to UV light Od. The cell does not contain enough nucleotides to make two complete strands Oe. Both B&D
Lagging strand: is synthesized discontinuously in the replication fork. direction toward the N terminal to the C terminal From the nitrogenous base to the phosphate group 5-> 3' 3'-> 5 direction toward the Leading strand: is synthesized continuously in the replication fork. N terminal to the C terminal From the nitrogenous base to the phosphate group 5-> 3' O 3'-> 5' How would the DNA sequence GCTATA be transcribed to MRNA? GCUAUA CGATAT CGAUAU O GCUTUT What bases would be found on the other strand of the DNA sequence GCTATA ? GCUAUA CGATAT CGAUAU GCUTUT O O O O O OO OO
Which of the following statements is NOT TRUE about genomic DNA replication? O A DNA replication begins at the origin of replication and proceeds bi-directionally O B. New nucleotides can be added by the DNA polymerase only to the 3 end of the growing strand O. Synthesis of new DNA is catalyzed by the enzymes called DNA polymerases OD. DNA replication is semi-conservative OE. DNA replication proceeds continuously on both parental DNA strands.
Which of the following is correct about the function of DNA Ligase? DNA ligase is used on the leading strand and it joins any disconnected strand together O DNA ligase is used on the lagging strand which helps in joining fragments of the strand together O DNA ligase is used on the lagging strand and it aids in correcting mistakes on this specific strand O DNA ligase is used to maintain the replication fork structure
A seiee hserved under a microscope, researchers observe that the DNA is able to be SE be replicated. Based on this observation, which protein involved in DNA replication is most likely mutated? To answer the question please: I) draw a scheme of DNA replication; 2) name the proteins that are required for DNA replication; 3) propose the consequences of the process.
In the mechanism of replication the O DNA leading strand is read from 3' to 5 O DNA leading strand is read from 5' to 3 O DNA leading strand product is synthesized 3' to 5 O DNA lagging strand is read from 5' to 3 O Only the DNA leading strand is read D Question 18 What factor is used repeatedly for lagging strand DNA replication but not repeatedly for leading strand replication? O ANTP's O DNA polymerase 3 O a parental DNA template O metal ions O a ligase