How do we prepare smear

Q: A glass flask is rinsed and autoclaved after use. It was then filled with sterile media in a sterile…

A: Introduction Microbes or microorganisms may be found on almost every surface, in the air we breathe,…

Q: what is the specific antimicrobial mechanism for mouthwash, hydrogen peroxide, isopropanal alcohol,…

A: Introduction  Bacteria can be divided into 2 categories on the base of their ability to form spore.…

Q: Do you think UVC disinfection of N95 face masks is effective? Give two important considerations for…

A: At the time of Covid-19, N95 masks are currently considered critical supplies by the centers for…

Q: - Give two reasons for heat fixing a smear before staining.

A: Heat fixing denatures bacterial enzymes, preventing them from digesting cell parts, which causes the…

Q: Select all methods that can be used disinfecting medical devices. Thermal UV Chemical Steam…

A: Disinfection is the process of  eliminating the pathogenic organism from the surface except some…

Q: ame of Organism: Staphylococcus saprophyticus Type of Medium Blood Agar Color Before Inoculation…

A: Beta haemolysis represents an entire breakdown of the haemoglobin of the red blood cells within the…

Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…

A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…

Q: What is the importance of an antimicrobial removal device (ARD)?

A: The antimicrobial removal device (ARD) is a device that removes antibiotic residues from blood…

Q: How to prepare smear and squash.

A: In general : Squash spreads were set up with little pieces of tissue estimating 1–2 mm size without…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: Which disinfectant is the most effective? and why? Which disinfectant(s) is (are) bactericidal? and…

A: Disinfectant A is most effective as  there is no growth of salmonella choleraesuis at dilution 1:2…

Q: 1. Give the method of sterilization of these laboratory apparatuses how they are sterilized;…

A: Method of sterilization: Inoculating loop:              Sterilized by poking it at an angle through…

Q: What would your plate look like if we did not use aseptic technique to place the antimicrobial disks…

A: The Kirby-Bauer disk diffusion test is utilized to determine the susceptibility of a pathogenic…

Q: uestions What is the purpose of the soft agar? 1

A: The questions ask about the role of soft agar.

Q: Inoculation Positive Results Negative Purpose Results Test Medium Reagents Mannitol Salts Loop Agar…

A: Biochemical reactions aid in identification of different bacterial species based on their…

Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…

A: Sterilization of following- petri dish - By using autoclave or by a series of steps that includes…

Q: Clearly define the terms sterilization, disinfection, decontamination, sanitization, antisepsis, and…

A: Hygiene stands for some practices that are performed to preserve health. Most of the microbes, which…

Q: I Why does't a negative stain colorize the cells in the smear?

A: Staining is a technique which is used for enhancement of contrast in samples mostly at the level of…

Q: How can I tell if there is contamination?

A: RFLP is a type of DNA polymorphism that has DNA sequence variations that can be recognized by…

Q: Area of no growth around an antibiotic disk indicates_________________.

A: Antibiotics are the substances that are produced synthetically or semi-synthetically from…

Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…

A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…

Q: selective agar media for salmonella typhi?

A: Selective agar medium is used to isolate a particular type of bacterial species or genus .

Q: What is the difference between cleaning, sanitizing, and disinfecting?

A: Cleaning, sanitization and disinfection are the process of making an area free from all the visible…

Q: Oven sterilization of aqueous culture medium. Effect: Rationale:

A: Oven sterilization utilizes dry heat of about 160 or 170 degrees celsius for one hour or 30 minutes,…

Q: Eosin methylene blue agar differential or selctive ? What are the ingridients/ chemicals make it…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: What are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a…

A: Introduction A smear is a small amount of culture spread in a very thin film on the surface of the…

Q: High temperatures ___________ and low temperatures __________ .a. sterilize, disinfectb. kill cells,…

A: Cells are the basic structural and functional unit of the organism. All living cells have cell…

Q: Differentiate between Gram positive and Gram negative cells in smears. 2. Discuss how to properly…

A: The Gram stain is the most important and commonly used staining technique in microbiology. It is…

Q: How does sterilizer spray work from drones?

A: Sterilization is a process by which a material is completely devoid of microorganisms like bacteria,…

Q: What advantages does alcohol have over hand soap for antisepsis of the skin?

A: Antiseptics are the chemical substances that inhibit the growth or kill the microbes that reside on…

Q: Do you think UVC disinfection of N95 face masks is effective? Give two (2) important considerations…

A: N95 filtering facepiece respirator or N95 mask is a piece of personal protective equipment that…

Q: List one method of disinfection, and one method of sterilization. Label which is which.

A: Disinfection is a process by which most of the harmful microbes are killed or inactivated. However…

Q: What will pose an ethical issue in these procedures are performed - mutilation, sterilization?

A: Sterilization is a procedure which is practiced to limit the family size in which removal or simply…

Q: On blood agar plate. What hemolysis is this? (alpha, beta, gamma)

A: Blood agar is both enriched and differential media that contains 5% of the sheep blood. Blood agar…

Q: What is the motivation behind the use of stab slant and surface inoculation

A: Agar slants are prepared by adding the agar media to the test tube and allowing it to cool in a…

Q: What is the antimicrobial removal device (ARD) blood culture system? Why is it important?

A: The blood culturing system helps in the identification of the pathogens that is present in the blood…

Q: how could you determine if there is any contamination on your agar?

A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…

Q: When a disinfectant or antiseptic's effectiveness is expressed as a phenol coefficient, a number…

A: Phenol coefficient:  It is a way to compare bactericidal (killing bacteria) nature of a chemical…

Q: Why don’t health departments routinely test for pathogens instead of using a sewage indicator?

A: The most widely used sewage indicator organisms are fecal coliforms. This is because these microbes…

Q: f an antimicrobial removal device (ARD) bl

A: Introduction- antibiotic removal device (ARD) it is used to extraxt residual antibiotics in blood…

Q: Should you sample an area for microbial growth before or after sanitization? Eplain why.

A: Sanitization is basically the instrumental process in which the microbes are usually killed by…

Q: Microbiological Examination of Non-sterile Products  1. The outer surface of the product container…

A: Sterilization is a process that means to free an object from all life of any kind. It is…

Q: why is it important that you disinfect your work area both before and after working with bacteria?

A: Bacteria are present everywhere in each and every corner,present on non-living surfaces and as well…

Q: Hi. I want to ask the question about the sterilization method? Which sterilization methods are used…

A: Sterilization is a process that is employed to kill or removes the microorganisms such as bacteria,…

Q: Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies…

A: Microorganisms are small organism that cannot be seen by naked eyes. Microorganism can be harmful to…

Q: List 2 non-chemical means of disinfection.

A: Disinfection refers to the process by which most pathogenic disease-causing microorganisms, except…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.