A. Experimental Results Sample No. 1 2 3 4 5 *Enter qualitative observations Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Colorless solution Aqua blue solution Table 2. Matching of Samples with Reaction Profile Rationale Sample No. Sample Identity 1 2 3 4 5 Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution

Basic Clinical Laboratory Techniques 6E
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ISBN:9781133893943
Author:ESTRIDGE
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Chapter6: Basic Clinical Chemistry
Section6.6: Blood Cholesterol And Triglycerides
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Qualitative Reactions of Carbohydrates: Matching of Samples with Reaction Profile

1. Obtain five unknown carbohydrates samples from your laboratory
instructor.
2. Place approximately 2 ml of each sample in separate test tubes.
Use fresh sample for each qualitative test.
a. For Barfoed's test, add 1 ml of the test reagent and heat the
samples in a water bath for 5 minutes. Observe and record the
change.
b. For Seliwanoff's test, add 1 ml of the test reagent and heat the
samples in a water bath for 5 minutes. Observe and record the
color change.
c. For Benedict's test, add 1 ml of the test reagent and heat the
samples in a water bath for 5 minutes. Observe and record the
color change.
d. For Lugol's test, add 1 ml of the test regent and mix well.
Observe and record the color change.
3. The samples used are the following: fructose, lactose, wheat flour,
table sugar, and ribose.
4.
Match the samples with the obtained reaction profile in Table 1.
State the reason(s) why you match each sample with a particular
reaction profile.
Transcribed Image Text:1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.
A. Experimental Results
Sample No.
1
2
3
4
5
* Enter qualitative observations
Sample No.
1
2
3
نيا
A
5
Table 1. Results of the Qualitative Tests of Carbohydrate Samples
Barfoed's Test
Aqua blue solution
Brick-red precipitates
Aqua blue solution
Brick-red precipitates
Aqua blue solution
Seliwanoff's Test
Colorless solution
Cherry red solution
Colorless solution
Colorless solution
Colorless solution
Benedict's Test
Rust-colored precipitates
Rust-colored precipitates
Aqua blue solution
Rust-colored precipitates
Aqua blue solution
Table 2. Matching of Samples with Reaction Profile
Sample Identity
Rationale
Lugol's Test
Brown solution
Brown solution
Blue-black solution
Brown solution
Brown solution
Transcribed Image Text:A. Experimental Results Sample No. 1 2 3 4 5 * Enter qualitative observations Sample No. 1 2 3 نيا A 5 Table 1. Results of the Qualitative Tests of Carbohydrate Samples Barfoed's Test Aqua blue solution Brick-red precipitates Aqua blue solution Brick-red precipitates Aqua blue solution Seliwanoff's Test Colorless solution Cherry red solution Colorless solution Colorless solution Colorless solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue solution Rust-colored precipitates Aqua blue solution Table 2. Matching of Samples with Reaction Profile Sample Identity Rationale Lugol's Test Brown solution Brown solution Blue-black solution Brown solution Brown solution
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Hi! Can you give an explanation for each sample identity in paragraph form (for the rationale)? Thank you.

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