Hey, I need help with this please: Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI, and BamHI sites. I have cut the plasmid with PstI and inserted a 4.0 kb fragment into the site. From the data below, I need to Produce a "restriction digest map" of the resulting plasmid. Please draw the "restriction digest map" with the given data and also explain the sequence of steps you took to construct the digest map so I can understand it thoroughly.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Hey, I need help with this please:
- Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI, and BamHI sites. I have cut the plasmid with PstI and inserted a 4.0 kb fragment into the site. From the data below, I need to Produce a "restriction digest map" of the resulting plasmid.
Please draw the "restriction digest map" with the given data and also explain the sequence of steps you took to construct the digest map so I can understand it thoroughly.
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