An enzyme has a Vof 1.2 uM sThe Km for its substrate is 10 µM.maxCalculate the initial reaction velocity, Vo, for each substrate concentration, [S].Calculate Vo when [S] is 2 µM.Vo =µM sCalculate Vo when [S] is 10 µM.Vo =µM s-1Calculate Vo when [S] is 30 µM.Vo =µM s-1 Graphical methods are available for accurate determination of the Vmax and Km of an enzyme-catalyzed reaction. However, thesequantities can sometimes be estimated by inspecting values of Vo at increasing [S].Estimate the Vmand Km of the enzyme-catalyzed reaction for which the data in the table were obtained.max[S] (M)Vo (uM min-1)2.5 x 10-6284.0 × 10-6401 x 10-5702 x 10-5954 x 10-51121 x 10-41282 x 10-31391 x 10-2140Please enter your answers using values from the table. Use a whole number for Vmax .

An enzymatic reaction is a biochemical reaction where the substrate is converted to product in the…

Answered: Graphical methods are available for…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2 μM substrate. At this substrate concentration, you determine that the Vo = 88.6 μmol mL-¹. s-¹. If the Vmax 833.3 mM s the KM is: . == " 30.9 μΜ 10.4 μΜ Ο 124.6 μΜ 234 μΜ 94.5 μM
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An enzyme catalyzes a reaction with a K of 7.50 mM and a Vmax of 4.15 mMs. Calculate the reaction velocity, o, for each substrate concentration. [S] = 1.75 mM MM-s-1 [S] = 7.50 mM [S] = 11.0 mM DO mM-s mM-s
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Enzyme A catalyzes the reaction S → P and has a KM of 50 μM and a Vmax of 100 nM s–1. EnzymeB catalyzes the reaction S → Q and has a KM of 5 mM and a Vmax of 120 nM s–1. When 100 μM ofS is added to a mixture containing equal amounts of enzymes A and B, which reaction product (Por Q) will be more abundant after 1 minute of reaction?

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