Given the following graph, what are the pKa's of the polyprotic species? Analytical Concentrations of Polyprotic Acid 1.0000 0.8000 0.6000 a1 0.4000 •a2 0.2000 a3 0.0000 0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 pH Fraction
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- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear…The decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the following rates were obtained. Temperature, °C Rate constant, s1 0.00 5.60 x 10-6 10.0 3.20 x 10-5 20.0 1.60 x 10 4 30.0 7.60 x 10-4 43. Fit this data into the Arrhenius equation. Give the values of the following: Slope = ( Select ) y-intercept = I Select] Pearson coefficient (R) = [ Select ] activation energy of this reaction in kJ/mol = [Select ] collision frequency factor A = I Select]
- The decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the following rates were obtained. Temperature, °C Rate constant, s 1 0.00 5.60 x 10-6 10.0 3.20 x 10-5 20.0 1.60 × 10-4 30.0 7.60 x 10-4 43. Fit this data into the Arrhenius equation. Give the values of the following: Slope = [ Select] y-intercept = [Select] %3DBased on information provided, could you please answer only question B? (Please show your work) Na2S203 Flask 1 Flask 2 Flask 3 Initial 14.15 28.04 Final 27.07 43.85 31.06 49.87 KBr03 Flask 1 Flask 2 Flask 3 Initial 40 35.20 41.2 Final 60 45.22 51.2 Concentration of Na2S203: 0.0898M Concentration of KBr03: 0.020M Concentration of NH3OhHCI: 0.02M Calculations a) For each of the three sets of data calculate the number of moles of KBr03 initially delivered into each flask, considering the volume of KBr03 delivered and its concentration. From the result of the titration of unreacted KBr03 with Na2S203, find the number of moles of KBr03 remaining in the solution at the end of the 10-minute reaction period. The difference between these two values gives the number of moles of KBr03 which reacted with the hydroxylammonium ion. Calculate the average of the three values for the moles of KBr03 which reacted. b) Find the number of moles of electrons which must have been transferred between Br03-…Which of the following is correct concerning the oxygenation plot of proteins X and Y shown below? Y (fraction saturation) 1.0 0.8 0.6 등 0.4 0.2 0.0 0 20 Oxygen binding plot 40 flog ha 60 p02 (torr) 80 100 X Y
- Penicillin is hydrolyzed and thereby rendered inactive by penicillinase, an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in 10 ml solution containing 10–9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change during the assay. [Penicillin] µM Amount hydrolyzed (nanomoles) 1 0.11 3 0.25 5 0.34 10 0.45 30 0.58 50 0.61 a) Plot V0 versus [S] and 1/V0 1/[S]. Does penicillinase appear to obey Michaelis-Menten kinetics? b) What is the value of KM? c) What is the value of Vmax? d) What is the turnover number of penicillinase under these conditions?Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Phosphate buffered saline (PBS) is a physiological buffer often used in biology experiments. Because the concentrations are very low and small amounts are difficult to weigh out, it is often prepared as a 10X stock solution meaning that it is prepared at 10 times the working concentration which is 1X. 1X PBS is made up of Na2HPO4 10 mM (MW 141.9 g.mol-1); KH2PO4 8 mM (MW 136.08 g.mol-1), NaCl 137 mM (MW 58.44 g.mol-1) and KCl 74.5 mM (MW 74.5 g.mol-1). You decide to prepare a 1 L 10X solution of PBS. What is the Molar (M) concentration of each of the components in you 10X stock. Write your answers in the table below. How much of each of the components should you weigh out to prepare 1L of the 10X stock solutions. Write your answers in the table below.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XWhat would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or tissue culture medium at pH = 7.95? Group of answer choices a. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); pK = 7.5 b. Histidine; pK1= 1.78; pK2= 5.97; pK3= 8.97 c. Bicarbonate; pK = 6.1 d. Phosphate; pK1 =2.0, pK2 = 6.8; pK3 = 12.5 e. Tris or Tris(hydroxymethyl)aminomethane; pK = 8.07Determine the correct check digit for the UPC of PH food prod: 1. 4-808887-03001- ?(purefoods corned beef) 2. 4-800088-11520-? (virginia spaghetti sauce)