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enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]

enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S k₁ k₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d(P) dt = K₂ [E]
Transcribed Image Text:An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S k₁ k₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d(P) dt = K₂ [E]
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