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Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50°C?
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- Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?Which is more Suitable for long-term storage: agar slant or plate? Explain why?A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…
- What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution to a sterile petri dish and then added molten agar to make a pour plate? O 1) 106 O 2) 10-7 O 3) 107 O4) 4) 10-5 O 5) 10-6You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then repeat this procedure two more times. What is the final dilution factor of this serial dilution? O 1) 10-4 O 2) 102 O 3) 10-6 4) 10-2 O 5) 104List five essential procedures of the aseptic technique when transferring a broth culture to another broth.
- Define agar.In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agarwhy do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
- In this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microfuge tube. Maintain constant agitation for 30 minutes at 4°C. Centrifuge in a microcentrifuge at 4°C. You may have to vary the centrifugation force and time depending on the cell type. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet. Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Once you have determined the concentration of each sample, you can freeze them at -20°C or -80°C for later use or prepare for immunoprecipitation or for loading onto a gel. Q4. Why is it necessary to store the prepared lysates in a very low temperature? PLEASE ANSWER Q3 AND Q4If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?Why should your solvent be evaporated from the sample placed on the sterile disk before you place it your petri dish?