Based on the TLC plate shown below, what was the error made? O The eluent used was too polar The sample spotted in lane 3 was too concentrated Multiple compounds were spotted in lane 3 O There is no error in this plate

Based on the TLC plate shown below, what was the error made? O The eluent used was too polar The sample spotted in lane 3 was too concentrated Multiple compounds were spotted in lane 3 O There is no error in this plate

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.17QAP: Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC...

See similar textbooks

Related questions

Q: What is the wavelength (in nm) of a photon if the energy is 7.41 x 10-19 J? (h= 6.626 x 10-34 J•s)

Q: Starting from the wedge-and-dash structure below, rotate the back carbon to provide the Newman…

A: Dihedral angle in Eclipsed conformation is zero degree and so this conformation have large steric…

Q: hy is it inappropriate to report yields with decimal-place accuracy (as opposed to whole numbers)?

A: Percent Yield of a reaction is always rounded of as a whole number and not with decimal place their…

Q: 16. Which of the following bases is the third strongest one? Which of the following bases is the…

Q: ЕНО N N. N р-аз РАЗРА N. OH

A: Functional group is atom or group of atom which gives special characteristics and identity to that…

Q: Balance each redox reaction in basic solution using the half reaction method. Cr₂O72- + Hg →→ Hg2+ +…

A: A question based on redox reaction that is to be accomplished.

Q: Naturally occurring chlorine is composed of two stable isotopes namely Cl 35 (75.76 %) and Cl 37…

Q: Is alcohol an acid or base?

Q: What is the mass of a cube of iron metal with sides that are 1.50 in in leagth if the density is…

Q: Please show how to calculate the J values for the proton NMR data provided.

A: In the given question we have to calculate the coupling constant (J value) of the following NMR…

Q: . Which of the compounds shown in the figure are amphoteric? I only Ill only C. I and II only d. II…

A: Amphoteric compounds are those which behave as acid as well as base, that is show dual behavior. For…

Q: When Neils Bohr created his famous model of the atom, what part of the scientific method was he…

Q: A d orbital essentially resembles: four p orbitals within the same atom two forbitals within the…

A: There are various orbitals such as p p orbital, s orbital, d orbital.s orbital is spherical in…

Q: What property of water causes pipes to break when they freeze? density of water high specific heat…

A: Here, we have to find the property of water that causes pipes to break when they freeze.

Q: KOH (concentration = 0.2205 M) is used in a titration to determine the amount of acetic acid…

A: Given : Initial volume of vinegar sample = 50 ml Volume of vinegar sample after dilution = 500 ml…

Q: Identify the oxidizing and reducing agent of the given equation: Answers should be the symbol of the…

Q: solution, find the volume (mL) of 0.9% NaCl solution. In 80 mL 5% dextrose in 0.9% Nach

Q: The concentration of oxygen at z=δ is 21 mole percent. The reaction at the surface may be assumed to…

Q: For what products is MinuteClinic a substitute?

A: We have to tell that for what products minuteClinic is a substitute .

Q: N O

A: Resonance structure is a collective representation of more than one lewis structure.It is also known…

Q: ol B HO 00 Of the compounds shown, which of the following would have the largest Rf value? O C A A…

A: Rf value I.e. retardation factor is distance traveled by solute divided by distance traveled by…

Q: The vapor pressure of water in a 1.00 mol/kg aqueous solution of urea at 20 degrees Celsius is 2.29…

A: This is a problem based on relative lowering of vapour pressure. The solution is given below.

Q: In redox reaction CIO is converted to CI in a basic solution. The balance have reaction for this…

A: The unbalanced redox equation is as follows: ClO- -----> Cl- O atoms are balanced by…

Q: A solution of permanganate contains 2.608 grams of KMnO4 per 750 mL. What is the normality of the…

Q: and D) have been discovered. These new elements undergo chemical reactions like the one shown below.…

Q: What is the solvent that is used to elute the TLC plate called? O Stationary Phase O Solvent Front O…

A: An eluting solvent seperates and moves component of mixture in column chromatography. Here we have…

Q: Why is sodium hydroxide used to test the solubility of phenol?

A: A question based on solutions that is to be accomplished.

Q: Calculate the temperature when ΔS(universe) equals to 0 for the following reaction: 2H2(g) + O2(g) →…

A: Given, ∆U = -564 kJ/mol I (work) = 7.5 kJ ∆S= 0 Reaction : 2H2(g) + O2(g) → 2H2O(l)

Q: 2 Predict the formula of the compound that form when lithium atoms react with nitrogen atoms.…

A: We know that, Atomic number of Li= 3 Atomic number of N=7 then,

Q: please show the mathematical workings for the solution

A: To determine the greatest volume, all the volume values will be converted to litres so that they can…

Q: A rigid 1 m³ tank contains nitrogen gas, initially at 10 bar, 27 °C. Heat transfer to the gas until…

A: The answer to the following question is-

Q: Provide the most stable(lowest energy) chair conformation of chlorocylohexane

A: We can draw the substituted cyclohexane in chair form which is stable form. There are axial and…

Q: 4.6 Precipitation Reactions: Exercise 4.6.2 Using the information in Table 4.6.1, predict what will…

Q: Need the following questions answered The reaction of interest:…

Q: A reaction catalyzed by a human enzyme was carried out at 20°c. If there is excess of substrate…

A: In this question, we have to choose the correct option regarding which would cause the greatest…

Q: A student reported the density for ten glass beads as d = 2.507 g / 1.22 mL = 2.054918033 g/mL…

Q: 2. Write down the electrode reactions is the following reference electrode, discuss under what…

A: The potential of an individual half-cell cannot be directly measured. But the difference between the…

Q: Consider the hypothetical reaction A+B+ 2C 2D + 3E where the rate law is A[A] At Rate == = k[A][B]²…

A: A question based on kinetics of solution that is to be accomplished.

Q: A. Supply the necessary arrows in the following equations. DON'T FORGET: a. b. C. d. H- HICI H H…

A: Here we are required to show the movement of electron from one atom to another atom.

Q: An oceanographer creates a model of a wave tank with a sand table to simulate beach erosion. What…

A: Here, the oceanographer creates the model to simulate beach erosion.

Q: Determine the ionic strength of a minimal growth medium that has the following composition: 0.150 M…

Q: 26 mg of sodium. How many atoms of sodium are found per mL of the solution? 1 oz. = 29.57 mL…

A: 1 oz = 29.57 mL

Q: the angular momentum the electron number the electron configuration the electron spin

A: There are four types of quantum numbers first is principal Quantum number (n) second is as a angular…

Q: The periodic table is the most important organizing principle in chemistry. There is a regular…

A: A question based on periodic table that is to be accomplished.

Q: Assume that all solutions are prepared at 25 degrees celsius and the solutes arr completely…

A: A beer contains about 5.50% (v/v) of ethanol that is 100 ml of beer contain 5.50 ml ethanol. So,…

Q: Use stoichiometric methods to analyze a mixture. A mixture of CaCl₂ and inert material is analyzed…

A: Here we are required to find the percentage of calcium present in the sample.

Q: what is the product after these compumds undergo a diels-alder reaction

A: Diels-Alder reaction is [4+2] cycloaddition reaction. In this reaction diene and dienophile reacts…

Q: H3C. C H2 H3C | CH HU H2C H2 C C OI CH2 CH3

A: A compound is a substance formed when two or more chemical element are chemically bonded together.

Q: Ammonium carbamate, NH4OCONH2, decomposes to produce ammonia and carbon dioxide. At 31.00 °C the…

Q: Give the name and the symbol for the amino acid H₂N-CH- CH-CH₂ CH₂ OH name: three-letter symbol:

A: Note : According to our guidelines we are supposed to answer only one question. Kindly repost other…

Question

Based on the TLC plate shown below, what was the error made?
The eluent used was too polar
The sample spotted in lane 3 was too concentrated
Multiple compounds were spotted in lane 3
O There is no error in this plate

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

How can the selectivity factor be manipulated in (a) GC and (b) LC?
Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC system to a mass spectrometer is a much more difficult task. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC with mass spectrometry.
A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
HPLC VFA analysis - why do I get so different peaks for very similar samples?
2. In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.
A chemist prepared a reagent blank and 6 Fe standards with the following concentrations: 20, 30, 40, 50, 60, and 70 ppm. The table below shows the average reading of each run in the UV-Vis spectrophotometer. Concentration (ppm)-- --Absorbance (au) -0.121 20- 30--- -0.302 40- -0.468 50- -0.573 60- --0.696 70--- -0.845 Blank- -0.04 Which of the following is the correct equation for the calibration curve? (A) y = -0.1700 x + 0.01402 (B) y = 0.01402 x -0.1700 Cy=0.01402 x -0.1300 (D) y=-0.1300 x + 0.01402
Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?
The ppm concentration of Pb2+ in a blood sample were measured with Spectrophotometry. 5.00 mL of a blood sample were taken and this sample gave a signal of 0.301 a.u.. Another 5.00 mL of a blood sample were mixed with 0.50 mL og 1.75 ppm Pb2+. Then, this mixture was diluted to 25.00 mL and this diluted mixture gave a signal of 0.406 a.u.. What is the ppm concentration of a blood sample?
A chemist prepared a reagent blank and 6 Fe standards with the following concentrations: 20, 30, 40, 50, 60, and 70 ppm. The table below shows the average reading of each run in the UV-Vis spectrophotometer. Concentration (ppm)-- -Absorbance (au) 20- -0.121 30- -0.302 40- -0.468 50- -0.573 60- -0.696 70 -0.845 Blank- -0.04 Which of the following is the correct equation for the calibration curve? (A) y = -0.1700 x + 0.01402 B y = 0.01402 x - 0.1700 C) y = -0.1300 x + 0.01402 (D) y = 0.01402 x - 0.1300
What is the Rf value given the following information: Height of Chromatography Paper: 5.0 cm Distance from Bottom of Paper to Origin: 1.5 cm Distance from Origin to Spot: 1.6 cm Distance from Origin to Solvent Front: 2.9 cm Your Answer: Answer
Polarities of analyte functional group increase in the order of hydrocarbon < ethers < esters < ketones < aldehydes < amides < amines < alcohols Arrange the order of elution [from shortest to longest retention time] for the following six compounds from an HPLC C18 column. C2H5OH CH3COCH3 CH3COOH CH3COOC2H5 C2H50C2H5 (СН3)2NH O A. C2H5OH < CH3COOH < (CH3)2NH < CH3COCH3 < CH3COOC2H5
Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L). Data for YELLOW: peak absorbance used on spectrophotometer =420nm Cuvette 1 - 100% dye = 0.680 absorption Cuvette 2 - 75% dye = 0.510 absorption Cuvette 3 - 50% dye = 0.340 absorption Cuvette 4 - 25% dye = 0.170 absorption Data for RED - peak absorption used on spectrophotometer = 500nm Cuvette 1 - 100% dye: 0.621 absorption Cuvette 2 - 75%: 0.466 absorption Cuvette 3 - 50% dye: 0.311 absorption Cuvette 4 - 25% dye: 0.1555 absorption