Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences? Explain briefly
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1. Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and
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- What makes platinum replica EM more desirable than conventional SEM and TEM (address both, specifically) for imaging cytoskeleton?1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain?b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.In what situation(s) would the use of a scanning electron microscope be ideal, and why?
- Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?1.) What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign out
- In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?Discussion Give the main differences between the types of microscopes used to diagnose surfaces?) What is the appropriate device to diagnose each of the following and why? O A- The aluminum plate is coated with a nano-solution. b- A colloidal sample containing nano-solutions. C- Study of the microstructure of the surface of a sample of iron.' Is it possible to use an optical microscope to study painted metal) surfaces? And why?4) What is the advantage of cryo-sectioning for antibody staining methods? 5) When preparing coverslips with cultured cells for immunofluorescence why is it necessary to coat coverslips with poly-L-lysine or gelatin?
- a) What are the important parameters for the eluent selection in Gel Permeation Chromotography (GPC)? Explain your answer in detail. What is an universal calibration curve and what is the advantage of using it in GPC analysis?What is the significance of UV unwrapping in the texturing process of 3D models?In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average value