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Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
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- use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?My professor instructed me to make presentation on this topic "Periodontal microbes interaction with FISH". I have to make 4 power point slides regarding this topic. Can you give some information about the topic which i can use in the slides? Also provide some necessary relevant diagram or image regarding the topic. Please answer at your own easy words. If you do so i will rate you positive, Thank you.
- Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
- The colony forming units (CFU)/ml in sauerkraut brine are given for different types of bacteria except for leuconostoc (shaded column). To obtain the Leuconostoc counts for different days, 40 microlitre of sauerkraut brine was 10-fold diluted and the CFU was determined by plating different dilutions on agar plates. For Day 0 56 CFU were counted in 10-1 dilution, Day 1 81 CFU were counted in 10-3 dilution, Day 2 86 CFU were counted in 10-3 dilution Day 5 78 CFU were counted in 10-5 dilution Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculationSearch ew View Help O Editing v BIUQv Av A ニv三マ 川 ... permitting the growth of gram positive bacteria. Is phenylethyl alcohol agar a differential or selective media? Selective media 5. A strain of Moraxella catarrhalis is able to grow on agar containing the antibiotic amoxicillin. You suspect this strain may contain the enzyme B-lactamase which inactivates amoxicillin, making it ineffective as an antibiotic. You grow the same strain in media containing amoxicillin and clavulanic acid (a B-lactamase inhibitor). What do expect the results to be; growth or no growth? Explain. s: On ENG 103 Project 1...pdf PDF Getting starte Personal Narrative..pdf PDF PDFSEPARATION OF COMPONENTS BY CENTRIFUGATION Consider the following subcellular components and their corresponding sedimentation rates. Table 3. Sedimentation rates of four subcellular components for isolation. CENTRIFUGAL FORCE (xg) SUBCELLULAR COMPONENT CDCP 14896 AJRA 967 AGC 382 JCKSP 2378 Two centrifuge machines are available in your laboratory. Centrifuge A has a rotor diameter of 14 cm and a maximum speed of 3500 rpm while centrifuge B has a rotor diameter of 18 cm and a maximum speed of 14500 rpm. Construct a separation scheme of the four subcellular components considering the following parameters: The four components are separated into pellet/supernatant I and pellet/supernatant II. Only two centrifugation steps are performed; specify the centrifugal speeds (in rpm) per step. Show relevant calculations. Both centrifuge machines must be used in the procedure. Components of each fraction are indicated.
- 1). SIM Tubes and TSI slants turn black if the organism is able to reduce iron compounds. For which organism(s) below would you expect a black reaction? Circle all that apply. Escherichia coli. Proteus vulgaris. Salmonella typhimurium. Alcaligenes faecalis. Shigella flexneri. 2). If Citrate is a sugar and fermentation generates an acid, why does the BromThymol Blue indicator in the media turn blue and not yellow? 3). Why is Salmonellosis associated with eggs? Can you get Salmonellosis from other animal sources?What results on a microdilution antimicrobial susceptibility test would indicate an antibiotic would be an effective treatment for an infection? O a. Growth next to the antibiotic O b. High optical density in the well with inoculum and antibiotic O c. Low optical density in the well with inoculum and antibiotic O d. No growth next to the antibiotic disk Clear my choice Next pageAntibiotic Code Zone of Inhibition (mm) S, I, or R? Penicillin P 15 Streptomycin S 8 Ampicillin A 25 Chloramphenicol C 14 Bacitracin B 5 QUESTIONS: Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain. 4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these…