An enzyme-catalyzed reaction was carried out in 5.0 mL of 0.15 M phosphate buffer pH 7.2. As a result of the reaction, the pH of the solution increased to pH 7.4. How many moles of OH- were produced during the reaction? H2PO4- + H2O ⇆ HPO42- + H3O+, pKa = 7.2

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Acid Base Titration

An acid-base titration is the method of quantitative analysis for determining the concentration of an acid and base by exactly neutralizing it with a standard solution of base or acid having known concentration.