Absorbance (arb.units) 2: 04 NO B-Carotene Calibration Curve 2004 6503 UM O 3.26 mM 5,06 nM. y (105612 M)x-0.1736 9294M 404 104 FRE 181 1211 Concentration (M) 1004 à Abserbence Carbunits) 0 0.25 200 A student creates a standard curve of B-Carotene and plots the line of best fit. They then measure the absorbance of a solution of B-Carotene with an unknown concentration. What is the concentration of the sample? You can assume they used a cuvette with a pathlength of 1 cm. 500 Wavelength (nm)

Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions
Section20.3: Uv-visible Spectroscopy
Problem 20.5P
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Absorbance (arb.units)
SA
8.3
B-Carotene Calibration Curve
2004
65,03UM
3.26 mM
5,06 nM
y=(105612 Mx-0.1736
2.94 M
4304 1064 ENY 181 121
Concentration (M)
1004
5
Abserbence (arb.units)
200
0.25
300
A student creates a standard curve of B-Carotene and plots the line of best fit. They then measure the
absorbance of a solution of B-Carotene with an unknown concentration. What is the concentration of
the sample? You can assume they used a cuvette with a pathlength of 1 cm.
400
500
600
Wavelength (nm)
YOU
Transcribed Image Text:Absorbance (arb.units) SA 8.3 B-Carotene Calibration Curve 2004 65,03UM 3.26 mM 5,06 nM y=(105612 Mx-0.1736 2.94 M 4304 1064 ENY 181 121 Concentration (M) 1004 5 Abserbence (arb.units) 200 0.25 300 A student creates a standard curve of B-Carotene and plots the line of best fit. They then measure the absorbance of a solution of B-Carotene with an unknown concentration. What is the concentration of the sample? You can assume they used a cuvette with a pathlength of 1 cm. 400 500 600 Wavelength (nm) YOU
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