A student was observing a filament of blue green bacteria under low power (60X). The diameter of the field is 2.4 mm. At this magnification, the student was able to count 40 cells in a row across the middle of the field of view. a. What would be the average length of each cell? b. If the student switched to high power (600X), about how many cells would be seen
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- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is 2m long. What is the magnification factor of the cell on the screen? 1 000x 4 000x 10 000x 40 000x 100 000x 400 000x
- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.If a slide has 28 yeast cells in a field of view on 100x, approximately how many yeast cells will be visible when the magnification is increased to 400x?A microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated B. the microbiologist put too much inoculum on the culture medium C. the medium was a selective medium D. the medium was differential
- You are viewing a bacterial cell that is 0.4 um in length and width using the 100X objective on your microscope with yellow light of 600 nm. 6. What is the limit of resolution? Express this value in nm and um. Will you be able to see an individual bacterial cell? Why or why not? a. b. 1001TWhat is the difference between total magnification and objective magnification? You are looking at an organism on the 20x objective What is the total magnification? If the organism takes up 62 units on the ocular micrometer, how big is the organism is micrometers? Show your work.You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and counted 67 cfu. What was the cell density of your bacterial culture (in cfu/ml? How many cells did you have in total if the volume of your culture was 50ml? Round to a whole number, do not write in scientific notation. The cell density of my bacterial culture was cfu/ml. The total number of cells was
- A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below. b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown resultsYou may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…