A peak elutes from an HPLC column 17.4 cm in length in 11.4 min. What would be the width at half-height of the peak (in seconds) if the plate height were 4.66 µm? W 1/2 = S
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- A peak elutes from an HPLC column 13.8 cm in length in 14.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were 7.75 µm? W 1/2 = What would be the width at half-height of the peak (in seconds) if the plate height were 46.0 μµm? W 1/2 = S SMany ions form colored solutions when dissolved in water. For example, aqueous Cu2+ solutions are light blue and aqueous Ni2+ solutions look light green. These metals in particularare often found in stainless steel. Would you expect their presence in your samples to interfere in the spectrophotometric analysis of permanganate at 525 nm? Why or why not? I think the answer is that I would expect the Cu2+ in my samples to interfere but not Ni2+ because the Ni2+ is green, signifying a higher wave length that the spectrometer wouldn't be able to pick up on because it was only set to 525 (blue-ish) where as the green is 530-ish. I just want to make sure thanks!Find the [FeSCN^2+]eq ε=4.0×10^3 M-1 cm^-1 at 447 nm
- Indicate the relationship that exists between the transmittance expressed as a percentage, T(%), and the absorbance, A.(1). A = - log [1/T(%)] (2). A + log T(%) = 2 (3). A = [log T(%)] / 100 (4). A = 100 log T(%)The validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choice1. A compound has a molar absorptivity of 3.031 x 10° L cm1 mol1. If a 15.00 mm-length cell is used to hold the sample, and a transmittance of 19.53 % is measured, what is the molarity of the compound in solution?
- A peak elutes from an HPLC column 13.8 cm in length in 14.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were 7.75 µm? W 1/2 4.1 W 1/2 Incorrect What would be the width at half-height of the peak (in seconds) if the plate height were 46.0 µm? 24.3 Incorrect S SIn the process of performing a spectrophotometric determination of Fe, an analyst prepares a calibration curve using a single-beam spectrometer, such as a Spec-20. After preparing the calibration curve, the analyst drops the cuvette used for the method blank and the standards. The analyst acquires a new cuvette, measures the absorbance of the sample, and determines the %w/w Fe in the sample. Will the change in cuvette lead to a determinate error in the analysis? Explain.Find the concentration of a solution with a transmittance of 0.27 AU and molar absorptivity of 3.65 x 104 M- cm in a 1.00 cm cell.
- A liquid sample was analyzed for Fe³+. A plot of absorbance (y-axis) vs. concentration (ppm; x-axis) of the external standards produced a calibration line with an equation y = 0.2256x -0.0903 (R² = 0.9973). The absorbance of the sample was 1.25. Determine the amount of Fe³+ in the sample (in ppm).Q4) (a) Explain how the components of a mixture are separated in gas chromatography. (b) An aqueous solution whose concentration is 0.00356 M was analyzed using spectrophotometer with a molar absorptivity of 412 cm-1 mol-1 L in a cell with a 2.00 cm pathlength. Calculate the absorbance and transmittance for the above solution. (c) Analyze the reasons why a dropping mercury electrode is better as compared to a conventional electrode.For the determination of Fe3 +, 25 mL of sample taken from sea water is diluted to 100 mL and a quantity of the obtained solution is taken and Fe3 + is determined by atomic absorption spectrophotometer. The absorbance values of the standard and sample solutions are below, and calculate the Fe3 + concentration in sea water in mg / L by drawing the relevant graph.Solution Absorbance1 mg / L Fe3 + 0.04095 mg / L Fe3 + 0.205110 mg / L Fe3 + 0.411615 mg / L Fe3 + 0.620820 mg / L Fe3 + 0.8222Sample Solution 0.5155