(a) From the information given in the table, calculate the specific activity of the enzyme, total yield (%), multi-fold increase in purity, and total multifold purification after each purification procedure,
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- Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…Separation of Amino Acids by Thin Layer Chromatography Lab Questions 1. Describe in detail Thin layer chromatographic experiment. Example: the theory behind it, how youwould prepare the materials to spot on the plate with different mobile amino acids and unknown andhow TLC Plate is developed and the reasoning behind which solvent/ solvent mixture should be used,along how to correctly identify of the unknown. 2. Calculate the Rf value if a solute travelled 5 cm from the base spot and the solvent front is 10 cmfrom the origin? 3. In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted thedevelopment of spots in the origin, what can you suggest about this observation?question: What is the purpose of adding table salt into the solution? What reagent is used in research labs/ Is it also table salt (NaCl)?
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.me Lab Evidence-Bas X NC Grades for Beatrice Pierre: 2024 X ourses/192624/quizzes/1488062/take Question 1 Background: While completing the enzyme lab exercises, you learned about the enzyme called catalase, which catalyzes the decomposition of H₂O2 hydrogen peroxide to O₂ (oxygen) and H₂O (water). You have performed experiments to investigate the effect of the following three conditions Substrate concentration, temperature, and pH. During the lab, you generated data, graphed your results, and made general statements about your results. Drawing Evidence-Based Conclusions: Write an overall evidenced-based conclusion about catalase that answers the question below. Your response should include supporting evidence from all of your data (3-4 sentences). Edit View Insert Format Tools Table B I U b My Questions | bartleby What effect did substrate concentration, temperature, and pH have on the activity of catalase? 12pt 3 pts Q Sea Paragraph x(A) Below is a partial purification table for a first attempt at a purification schedule for an enzyme: Technique Initial IMAC IEX Gel Filtration Protein (mg) 3467.8 196.7 153.6 142.1 Enzyme activity (units) 36489 32575 27891 26398 Overall yield (%) 100 Specific activity (units/mg) 29.46 Complete the table below by calculating the missing values. Overall Enrichment 1.00
- 18YW9TJVEYLM/edit of Enzyme Inquiry Questions mat Tools Add-ons Help Last edit was 3 days ago Normal text Calibri 13.5 BIUA GD 1. 3 . 4 E E E | Enzymes Narme: Directions: Each series of diagrams is designed to provide you with information about enzymes and chemical reactions. Please highlight, color, and/or BOLD your responses to make them easier to read! (6.5 pts/ question) Reactants Diagrams 1-4. Chemical reactions involve changes in chemical bonds. A CHEMICAL REACTION Product (left side of the arrow) (right side of the arrow) reactants - products NH, + HCI > NH,CI chemical reaction starts with one set 4. of molecules and turns them into Producte- he Reactante.the starting molecules 2H, + O, - 2H,0 final molecules something new by forming or breaking 6CO, + 6H,O - C,HO, + 60, chemical bonds. Reactants (Substrates) Products Chemical change 1. Explain what the "reactants" are in a chemical reaction. 2. What does the "arrow" of a chemical reaction represent? 3. What are the molecules…Protein Agarose Gel Electrophoresis Objectives: At the end of the exercise, you should be able to1. Understand the concepts of protein structures, isoelectric point of amino acids andproteins.2. Understand the principle of separation in protein agarose gel electrophoresis.3. Demonstrate laboratory techniques used in running a protein agarose gel (preparinggels, loading samples with micropippets, and electrophoresis).4. Understand the molecular difference between normal and mutant forms of betahemoglobin in patients with sickle cell disease, and how protein agarose gelelectrophoresis can be used in disease diagnosis. Comparing Migration Rates of Hemoglobin Isolated from Normal, Heterozygousand Sickle cell anemia Individuals at pH 9.2: Part B: pH 9.2, 1X buffer Materials on the supply bench:1) gel running apparatus (lid matches the tank); one comb.2) flasks for preparing agarose gel (125 ml)3) micropippets (P20) and yellow tip boxes4) plastic waste cup, markers and microcentrifuge…Biomaterial question: Give a brief and clear answer, please a. Briefly describe the structural differences between the three major types of biomaterials, including their chemical structure, bonding characteristics, crystal structures, etc. b. Explain briefly the working principle and the information provided by the spectroscopic and chromatographic characterization techniques . Give an example to each of them.
- Rf Sample values 0.21 В 0.39 C 0.33 0.27 Four amino acid samples were subjected to a normal-phase chromatography set up yielded the following Rf values. Which among the samples is the least polar? E and O A O B ODBIOCHEMIO TRY PRE-ACTIVITY Q: Given that BSA has a molecular wigntof s0,000 Daltons (9imol) please calculate the.concentration JOf a solution con toIning 10 BSA In.15 UL water in the followin concentra ton units Mol arity LuM) gram V. Mass. Rer volume % wlv C1. weignt volume)Active Raw Actual Yield Formulation Dosage Form Packaging Ingredient Materials 2 kgs calcium oxide and Calcium 1.75kgs 16omg per Oral 100mL per carbonate calcium 10mL Suspension bottle 1.75 kg H,O carbonate suspension Please fill the cells in the second column of the table below with correct information. Thank you! IA. RAW MATERIAL SOURCING Source (natural/synthetic) of RAW MATERIALS Method of separation/chemical reaction (with brief process) IB. ACTIVE INGREDIENT INFORMATION Active Ingredient Chemical Name/Other Name Active Ingredient Classification (ex. Analgesic)