A Count CD90 APC-A B MSC canine CD29/45/90-BM02/CD29/45/9 CD29+ IgG CD90+ IgG Count 75 de C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21 Count 150 CD29 PE-A 001 10 CD45 FITC-A IgG CD45+ Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+ (77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.
A Count CD90 APC-A B MSC canine CD29/45/90-BM02/CD29/45/9 CD29+ IgG CD90+ IgG Count 75 de C MSC canine CD29/45/90-BM02/CD29/45/91 D MSC canine CD29/45/90-BM02/CD4/8/21 Count 150 CD29 PE-A 001 10 CD45 FITC-A IgG CD45+ Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+ (77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
explain this result in a brief and clear way, Here is the link where the image came from: https://mdpi-res.com/d_attachment/ijms/ijms-21-05129/article_deploy/ijms-21-05129.pdf?version=1595262192
![3 de 21
2. Results
2.1. Flow Cytometry with Canine CD Markers
Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of
surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical
for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3
expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was
low (3.0± 1.7%).
Int. J. Mol. Sci. 2020, 21, 5129
A
C MSC canine CD29/45/90-BM02/CD29/45/91
CD90+
-IgG
CD90 APC-A
B MSC canine CD29/45/90-BM02/CD29/45/9
CD29+
HAKA
Count
10
CD29 PE-A
D MSC canine CD29/45/90-BM02/CD4/8/21
10
10
CD45 FITC-A
IgG
CD45+
3 of 21
Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note
confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses
of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+
(77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3280083f-2fe2-4ad0-ad93-2c58ae11f54a%2F7567c17b-9ba0-49fb-adff-144ea73d65d8%2Fhd3fn0jd_processed.jpeg&w=3840&q=75)
Transcribed Image Text:3 de 21
2. Results
2.1. Flow Cytometry with Canine CD Markers
Flow cytometry analyses confirmed that the population of BMMSC expresses a low number of
surface markers typical for hematopoietic cells (CD45), while expression of surface markers typical
for MSCs (CD29 and CD90) is high. The results presented in Figure 1 show that cells at passage 3
expressed primarily CD29 (88.5 ± 2.2%) and CD90 (77.3 ± 4.6%) and the proportion of CD45+ cells was
low (3.0± 1.7%).
Int. J. Mol. Sci. 2020, 21, 5129
A
C MSC canine CD29/45/90-BM02/CD29/45/91
CD90+
-IgG
CD90 APC-A
B MSC canine CD29/45/90-BM02/CD29/45/9
CD29+
HAKA
Count
10
CD29 PE-A
D MSC canine CD29/45/90-BM02/CD4/8/21
10
10
CD45 FITC-A
IgG
CD45+
3 of 21
Figure 1. Immunophenotyping of bone marrow tissue-derived MSC (BMMSC) at passage 3. (A) Note
confluent population of BMMSC revealing spindle-shaped morphology. (B-D) Flow cytometry analyses
of BMMSC expressing high percentage of mesenchymal markers CD29+ (88.5 ± 2.2%) and CD 90+
(77.3 ± 4.6%) and low percentage of hematopoietic markers CD 45+ (3.0 ± 1.7%). Scale bar A = 50 μm.
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