From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is:Q14nMΟ 10 μΜ-2 nM2 nMCalculate the Hill Coefficient from the receptor-ligand binding data below:Q2410023(0-1) 60log1086420-2-4-6-6-4-202 4log [L] (nM)6810

The hill plot is a graph drawn based on the Hill equation given below.This identifies with the…

Answered: 8 From the Hill Plot below, the of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is: Q1 4 nM 10 μΜ -2 nM 2 nM Calculate the Hill Coefficient from the receptor-ligand binding data below: Q2 4 100 2 3 (077) log 10 8 6 4 2 0 -2 -4 -6 -6 -4 -2 0 2 4 log [L] (nM) 6 8 10
The Graph below shows the binding curves of two proteins (A and B) for the same ligand (L). Use this Graph and determine the dissociation constant, K, for both proteins. Which protein (A or B) has a greater affinity for ligand L? Which of the two proteins would be more easily inhibited by an antagonist? 1.0 Y 0.5 2 A 4 6 B 8 [L] (μM) 10 12 14 16
Estimate the binding affi nity of a ligand for its receptor from the followingdata:
The dissociation equilibrium constant (Kd) is:_____ (select all that apply) Group of answer choices: Is a measure of ligand efficacy Typically equivalent to 1- Ka The concentration of ligand that gives maximal receptor occupancy The concentration of ligand that gives half-maximal receptor occupancy Measured in 1/M or M-1 Typically measured in mol/L or M A measure of the strength of a ligand - receptor binding reaction
added ligand concentration of 10 µM is 5 × 10³ s¹. What is the value of the reverse rate constant k_1 = 1 ×10-4 Incorrect Macmillan Learning You have not correctly calculated the value of the reverse rate constant. To begin, calculate the value of k₁ by dividing the rate of receptor-ligand complex formation (the rate of the forward reaction) by the ligand concentration. k₁ = rate forward [L] Then, calculate the value of the reverse rate constant, k_₁, by multiplying the dissociation constant, Kd, and the forward rate constant, k₁. k_1 = Kak₁
Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB? What is the equation for the double-reciprocal plot at 20 nMnM NAMB?
A ligand binds two different receptors with a Kd value of 10−7 M for receptor 1 and a Kd value of 10−9 M for receptor 2. For which receptor does the ligand show the greater affinity? Calculate the fraction of receptors that have a bound ligand ([RL]/RT) in the case of receptor 1 and receptor 2 if the con- centration of free ligand is 10−8 M.
Two co-op students at your start-up company have been asked to evaluate the rate of reaction occurring in a transparent gel particle containing immobilized mouse melanoma cells. The equation for the reaction rate ris is: Vmax T'As Km+CAs where R=3.2 mm, Vmax= 0.12 gmol s' m3, CAS = 41 gmol m3, and Km=0.8 gmol m3. One student reports a reaction rate of 1.6x10 gmol st; the other reports 1.6x10 1º gmol s1. You left your calculator on the bus this morning, but must know quickly which student is correct. Use an order-of- magnitude calculation to identify the right answer. Which student is correct?
2) After plotting actin bound to cofilin versus the cofilin concentration, you generate the figure below. v (cofilin binding density) 1 0.8 0.6 0.4 0.2 0 2 4 6 8 [S. pombe cofilin] free (UM) 10 a) What is the Kd you measure for this interaction? b) What is the AG of the interaction? c) Based on the data, is there anything you suspect about the binding of protein and ligand?
Doxorubicin is a chemotherapeutic drug with an IC50 in MCF7 breast cancer cells of 0.01 microM. Assuming the cytotoxicity of doxorubicin follows 'classic' receptor theory what percentage of the cells will be viable following treatment of the cells with a concentration of doxorubicin of 0.01 microM? O 10% O 0.01% O 90% 50% 99.99%
You are a technician in a biochemistry lab running receptor binding experiments. The target membrane-bound receptor has been partially purified from mouse, rat, and human cell lines. Using the same radioactive ligand in a saturation binding assay for each species' receptor, you generate the binding data in the table. The dependent variable, Y, is the fraction of binding sites occupied by the ligand. Ligand Y for mouse Y for rat Y for human concentration receptor receptor receptor (nM) 0.20 0.048 0.29 0.17 0.50 0.11 0.50 0.33 1.0 0.20 0.67 0.50 4.0 0.50 0.89 0.80 10 0.71 0.95 0.91 20 0.83 0.97 0.95 50 0.93 0.99 0.98 Determine the Ka for the human receptor in this binding experiment. human receptor Kd nM
During an SAR effort to identify tyrosine kinase inhibitors, it was found that compound 2 was significantly more potent and selective than compound 1. To what might you attribute this improvement in activity and selectivity? Explain. IC-5 micromolar IC-0.1 mromolar

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8 From the Hill Plot below, the of the first binding event for the receptor-ligand system under study is: 10 Q1 4 nM Ο 10 μ. -2 nM 2 nM (0-1) 601 6 4 2 -2 Calculate the Hill Coefficient from the receptor-ligand binding data below: Q2 -4 4 100 2 3 -6 -6 -4 -2 0 2 4 6 8 10 log [L] (nM)