6. Riboswitches are RNA sequences found in the 5' untranslated region of messenger RNA (mRNA) that control gene expression in response to small molecule binding. These regulatory elements adopt two conformations: an "on" state when a specific small molecule ligand is bound and an "off" state in the absence of ligand. When the mRNA is "off", the gene remains untranslated. The structure of a guanine-responsive riboswitch was solved in 2004 (Nature, 432, 411-415) and is shown below at low (left) and high (right) resolution with a guanine derivative bound: (see Blackboard for color image) Guanine riboswitch with a guanine derivative (red) bound; zoomed out. U22 U47 HX C74 U51 Guanine riboswitch with a guanine derivative (HX) bound; atomic detail. Note: blue = nitrogen, red = oxygen. C and U follow standard nucleotide naming conventions. a. What interactions are occurring between the riboswitch and the guanine derivative that make binding favorable? b. Of these interactions, which ones confer specificity for the riboswitch binding guanine and not the other nucleobases (C, A, T, U)?
6. Riboswitches are RNA sequences found in the 5' untranslated region of messenger RNA (mRNA) that control gene expression in response to small molecule binding. These regulatory elements adopt two conformations: an "on" state when a specific small molecule ligand is bound and an "off" state in the absence of ligand. When the mRNA is "off", the gene remains untranslated. The structure of a guanine-responsive riboswitch was solved in 2004 (Nature, 432, 411-415) and is shown below at low (left) and high (right) resolution with a guanine derivative bound: (see Blackboard for color image) Guanine riboswitch with a guanine derivative (red) bound; zoomed out. U22 U47 HX C74 U51 Guanine riboswitch with a guanine derivative (HX) bound; atomic detail. Note: blue = nitrogen, red = oxygen. C and U follow standard nucleotide naming conventions. a. What interactions are occurring between the riboswitch and the guanine derivative that make binding favorable? b. Of these interactions, which ones confer specificity for the riboswitch binding guanine and not the other nucleobases (C, A, T, U)?
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter15: Genes And Proteins
Section: Chapter Questions
Problem 18RQ: A scientist introduces a mutation that makes the 60S ribosomal subunit nonfunctional in a human cell...
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