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See attached, you have a table that is perhaps required for some of the questions.
Answer question number 4 in your own words. Do not copy and paste from google.
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- Help me answer question 3 4. Seliwanoff’s test – prepare 4 test tubes and place 5 drops of glucose, fructose, sucrose and xylose in separate test tubes. Add 1 ml of seliwanoff’s reagent in each of the 4 test tubes. Immerse in a boiling water bath. Observe the color change in each tube after 3 minutes of boiling. If no color change is observed, continue heating for another 2 minutes and observe.Review your data and calculations and complete the following discussion questions. 1. Explain how the slope of the line of absorbance vs. time provides information regarding protease activity. । ॐ EB EIT T Normal BIIUBARFOED’S TEST FOR MONOSACCHARIDESPlace 1 mL (20 drops) of each 1% carbohydrates solution in separate test tubes. Prepare a control with 1 mL water. Add 4 mL Barfoed’s reagent. Place the test tubes in boiling water bath for 10 minutes. Remove the test tubes and cool in cold water. Record your observations. A precipitate of cuprous oxide which may be green to red, indicates that a monosaccharide is present. Record your result and conclusion. Identify the compounds that are monosaccharides. SELIWANOFF’S TEST FOR KETOHEXOSESPlace 5 drops of 2% carbohydrate solution in separate test tubes. Prepare a control with 5 drops water. Add 1 mL of Seliwanoff’s reagent to each test tubes and heat. Record your results. The rapid formation of a bright red indicates that a ketohexose is present. Identify the compounds that are ketohexoses. IODINE TESTS FOR POLYSACCHARIDESPlace 2 mL of each carbohydrate solution in separate test tubes. Prepare a control with 2 mL of water. Add 1 drop of iodine solution…
- answer the following questions 1.What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer? 2.What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle?3.What is the optimum temperature for invertase reactions?4.What is the optimum ph for the invertase reactions?1. pH cannot affect the enzyme - activity A. True B. False 2. It took 6 mins for the disappearance of blue-black color in test tube 4 with 60 degrees Celsius temperature. A. True B. FalseHydrolysis of polysaccharidesa. Add 20 drops of concentrated HCl to 30 mL of 3 % starch solution. Cover the beaker containing the mixture with a watch glass and boil gently. b. After one minute addition of acid solution, remove 2 drops of the solution and place on a spot plate. Test each spot with one drop of iodine solution. c. Perform the same test using Benedict’s solution instead of iodine solution. Hydrolysis of polysaccharides Results Starch Solution Iodine's Test Benedict's Test
- I need help with Carbohydrate fermentation! Please indicate if each organism ferments the carbohydrate. indicate whether or not the phenol red indicator changes color and if it will produce gas. Organisms: Escherichia coli Alcaligenes faecalis Staphylococcus epidermidis Proteus vulgaris Carbohydrates: - glucose - lactose - sucrose Choose between the following results: Acid (yellow)/ no gas Acid (yellow)/ gas No acid (fuschia)/ gas No acid (fuschia)/ no gas.Test result shown in photo (+/-) The test demonstrates the presence or absence of which enzyme? Citrate کوه ها Urea PlasmaHydrolysis of polysaccharidesa. Add 20 drops of concentrated HCl to 30 mL of 3 % starch solution.Cover the beaker containing the mixture with a watch glass and boilgently.b. After one minute addition of acid solution, remove 2 drops of thesolution and place on a spot plate. Test each spot with one drop ofiodine solution. c. Perform the same test using Benedict’s solution instead of iodinesolution.
- What will you observe when the isolated beta amylase enzyme is subjected to biuret testWhich of the following statements best describe testing of lactose sample?* A. Lactose will quickly (less than 3 minutes) form a thin brick red precipitate in a Barfoed's test since it is a reducing monosaccharide. B. Lactose will give positive test using phenylhydrazine test and forms a needle shaped osazone C. Lactose when reacted with nitric acid and exposing to cold water bath will form insoluble white crystals. D. Lactose when tested using Seliwanoff's test will form a red colored complex.DESCRIBE THE RESULTS OF THE REDUCTION TEST FOR SUGARS USING THE FOLLOWING TEST PICRIC ACID TEST SUGAR RESULT 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. Galactose