4. A scientist cuts a large amount of produce a range of DNA pieces that are relatively similar in size. Scientists can vary the density of the agar gels by varying the amount of agar in the gel. Would she want to make an agarose gel with a higher or lower amount of agarose in order to resolve the DNA bands? A higher amount of agarose in the gel makes it stiffer. Explain.

4. A scientist cuts a large amount of produce a range of DNA pieces that are relatively similar in size. Scientists can vary the density of the agar gels by varying the amount of agar in the gel. Would she want to make an agarose gel with a higher or lower amount of agarose in order to resolve the DNA bands? A higher amount of agarose in the gel makes it stiffer. Explain.

Name: 4. A scientist cuts a large amount of DNA using a restriction enzyme
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Description: 4. A scientist cuts a large amount of DNA using a restriction enzyme that she believes willproduce a range of DNA pieces that are relatively similar in size. Scientists can vary thedensity of the agar gels by varying the amount of agar in the gel. W

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
1. Draw a gel showing the bands/fragments generated from cutting the plasmid below with Sall, BamHI and Pstl. Make sure you label the bands with the expected sizes. PstI 3607 3000 4000 amp ori HindIII EcoRI 4359 0 29 EcoRV 2295 1 NdeI 185 pBR322 4361 bp 375 tet 2000 BamHI 651 Sall 1000 2. If you want to use the plasmid above to clone an insert of 500 bp using BamHI and Sall, what would your expected FINAL plasmid+insert size to be if your cloning was successful? (remember that you first have to treat the intact pBR322 with BamHI and Sall to prepare it for introducing the insert).
2. Which of these statements is INCORRECT about restriction enzymes Restriction enzymes form phosphodiester bonds when DNA are assembled Palindromic sequences are recognized by restriction enzymes Restriction enzymes recognize blunt ends Restriction enzymes recognize sticky ends Restriction enzymes originated from bacteria
1. What are restriction enzymes and what do they do? 2. Complete the chart Original DNA sequence 5' TGA CCA CTC GAG CAT AAC GAG TCG CTC 3° Write the complementary strand's sequence * remember it will be opposite directionality (the number at the ends are different) * pairs are A-T and C-G |Copy and paste your double stranded DNA into this box. You are going to use the restriction enzyme Xhol. Mark the cut site by changing the color (highlight or change text color) of one double-stranded piece * it cuts between the C and the T (cut shown as slash symbol): 5’ C/TC GAG 3' * remember it cuts BOTH strands (the restriction site is palindromic). Hint: read each strand in the 5'à 3' directions to find the cut site
_1. Bacterial proteins that have the ability to cut both strands of the DNA molecule at certain points 2. Contain foreign DNA 3. Is made by connecting segments of DNA from different sources _ 4. General term for a carrier used to transfer a foreign DNA fragment into a host cell 5. A small ring of DŇA found in a bacterial cell 6. The procedure for cleaving DNA from an organism into small segments, and inserting the f. genetic engineering or segments into another organism a. recombinant DNA b. vector c. restriction enzymes d. plasmid e. transgenic organisms recombinant DNA technology
4. This is the set-up of our experiment. There are four plates shown: LB only (a type of nutrient agar), LB/AMP (LB agar with ampicillin included), and LB/AMP/Arabinose (LB agar with ampicillin and arabinose). All plates were spread with E. coli following the transformation procedure described in the video. Some of the E. coli samples were mixed with pGLO plasmid DNA (+DNA samples) and some E. coli samples were not (-DNA). Plate B -DNA Plate A LB LB/AMP Plate C Plate D +DNA LB/AMP LB/AMP/ARA 5. Complete the following chart to walk through the results you expect to see from the transformation procedure. 2 Based on B, IfE. coli coli added to coli ampicillin should the E. should grow, included as controls coli have grown on the glow? Did the E. Was the E. Plates A & B are the plate have resistant? the PGLO plasmid? (How do you plate? figure this out?) should it also for the lab. What do these plates tell you? (If no growth on plate A or growth on plate B) Plate A LB (general growth media)…
9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.
5. Show the separation pattern of the following DNA molecules on an agarose gel electrophoresis. 5 kbp 5 kbp 5 kb 5 kbp ----
9) The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)
4. A linear piece of DNA has the following EcoRI restriction sites. EcoRI site 1 2 kb 4 kb EcoRI site 2 5 kb a. This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
Question Image hown bele nallest? Q. A gel from gel electrophoresis is shown below. Which DNA fragment is the smallest? B. Direction of Travel
1. A plasmid was digested with two restriction enzymes, individually and in a double digest. The following fragment sizes (bp) were determined by agarose gel electrophoresis. Restriction Enzyme|Fragment Size (bp) 30, 15, 5 HindIII 50 EcoRI + HindIII 20, 15, 10, 5 Make a restriction map of the plasmid based on this data and indicate the distances between the restriction sites.