3. Which of the following statements best describes a non-specific assay? directly measures the concentration of an analyte indirectly measures the concentration of an analyte changes color as a function of analyte concentration measures the concentration of analyte using a absorption spectrometer
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- explain the results of this experimet Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088Sel Absorbance S Verify your molar absorptivity value. Did you report your data to the correct number of significant figures? Analytical wavelength Analytical wavelength for blue dye (nm) Calibration curve Solution 1 2 3 4 5 Dye concentration (μM) 15.20 7.51 Unknown solution 3.74 1.89 1.01 Molar absorptivity (μM ¹cm-¹) Absorbance Concentration of unknown blue dye (µM) 629 Absorbance 0.756 0.432 0.259 0.175 0.144 0.083 How to calculate concentration from absorbance S A d tra 0145.0 mL of an unknown FeCl3 solution is diluted to a total volume of 230.0 mL. The diluted FeCl3 solution measured an absorbance of 0.223. Calculate the molarity of the undiluted unknown FeCl3 solution if the molar extinction coefficient is 230.0 M-1⋅cm-1 and a path length of 1.00 cm. Group of answer choices 0.000190 M 0.00496 M 0.00593 M 10.0 M 0.00399 M
- q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762The concentration of methylene blue in the sample is estimated through (select the correct) options: refractive index measurement (refractometry). turbidity measurement (turbidimetry). absorbance measurement (spectrophotometry). titration with 2% HCl solution.Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
- If an analytical method relies on a measurable property of an analyte that can be correlated with the amount of the said analyte, what type of analytical method is it? Oxidative methods Instrumental methods Blank Correction methods Classical methodsData for finding log P of p-toluoyl penicillin Concentration of solution 1 is 0.001 M. The consequent solutions were prepared by two-fold serial dilution. Solution number Solution 1 Solution 2 Solution 3 Solution 4 Solution 5 Solution 6 Solution 7 Solution 8 Solution 9 Solution 10 Absorbance (342 nm) of octanol solution, initial 4 (overloaded) 4 (overloaded) 3.36 (too high) 1.658 0.831 0.482 0.268 0.173 0.086 (too low) 0.042 (too low) Absorbance(342 nm) of octanol solution, after shaking with water 1.650 0.810 0.452 0.253 0.154 Determination of logP from your data: Plot a calibration graph using initial absorbance data. Use this calibration graph to find concentrations of penicillin in octanol solutions after shaking them with water. The difference between initial and new penicillin concentrations in octanol solutions will give you amount of penicillin that moved into the aqueous phase in each case. Finally, calculate the log P for each solution using the following formula: logP = log-…Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A," following is correct? 715 at 257 nm". Which of the O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCI OC. An analyst can skip the shaking step O d. B andC O e. All of the above
- PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NAOH with HCI C. An analyst can skip the shaking step d. B and C e. All of the aboveBelow is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A, = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCl O c. An analyst can skip the shaking step O d. B andC O e. All of the above