3. Show that the inverse of equation 29-78 in the textbook gives the Lineweaver-Burk Equation. The hydration of CO2 is catalyzed by the enzyme carbonic anhydrase. For a total enzyme concentration of 2.32 x 10-⁹ M, the following data were obtained. [CO2]o (M) 0.00125 0.00250 0.00500 0.02000 v (M/s) 2.78 x 10-5 5.00 x 10-5 8.33 x 10-5 1.66 x 10-4 Plot these according to the Burke-Lineweaver Equation and determine the values of Km, the Michaelis constant, and k2, the rate constant for product formation from the enzyme-substrate complex from the slope and intercept of the plotted data.
3. Show that the inverse of equation 29-78 in the textbook gives the Lineweaver-Burk Equation. The hydration of CO2 is catalyzed by the enzyme carbonic anhydrase. For a total enzyme concentration of 2.32 x 10-⁹ M, the following data were obtained. [CO2]o (M) 0.00125 0.00250 0.00500 0.02000 v (M/s) 2.78 x 10-5 5.00 x 10-5 8.33 x 10-5 1.66 x 10-4 Plot these according to the Burke-Lineweaver Equation and determine the values of Km, the Michaelis constant, and k2, the rate constant for product formation from the enzyme-substrate complex from the slope and intercept of the plotted data.
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 9.4P
Related questions
Question
![3. Show that the inverse of equation 29-78 in the textbook gives the Lineweaver-Burk
Equation.
The hydration of CO2 is catalyzed by the enzyme carbonic anhydrase. For a total enzyme
concentration of 2.32 x 10-⁹M, the following data were obtained.
[CO2]o (M) v (M/s)
0.00125
2.78 x 10-5
0.00250
5.00 x 10-5
0.00500
8.33 x 10-5
0.02000 1.66 x 10-4
Plot these according to the Burke-Lineweaver Equation and determine the values of Km, the
Michaelis constant, and k2, the rate constant for product formation from the enzyme-substrate
complex from the slope and intercept of the plotted data.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff6ab806b-464c-4756-9d8f-72da0489465e%2F0acbd447-660d-465e-a4c7-3e3e913eb989%2F0zuv5v8_processed.png&w=3840&q=75)
Transcribed Image Text:3. Show that the inverse of equation 29-78 in the textbook gives the Lineweaver-Burk
Equation.
The hydration of CO2 is catalyzed by the enzyme carbonic anhydrase. For a total enzyme
concentration of 2.32 x 10-⁹M, the following data were obtained.
[CO2]o (M) v (M/s)
0.00125
2.78 x 10-5
0.00250
5.00 x 10-5
0.00500
8.33 x 10-5
0.02000 1.66 x 10-4
Plot these according to the Burke-Lineweaver Equation and determine the values of Km, the
Michaelis constant, and k2, the rate constant for product formation from the enzyme-substrate
complex from the slope and intercept of the plotted data.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 20 images
Recommended textbooks for you