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2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide.
3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color.
4. Discuss why human blood plasma will not always yield reliable results.
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- . A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…2. Superoxide dismutase is used to detoxify H2O2 and produce H2O. True or False.1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. 4. Discuss why human blood plasma will not always yield reliable results.
- 1. What results are expected if you drop H2O2 into a cut on your arm? Explain why those results are expected. 2. To do this test, why do you have to grow the organism in medium containing tryptophan? 3. Why is a red color after the addition of zinc a negative result for nitrate reductase?1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?1. Proteus vulgaris is positive for the production of the enzyme phenylalanine whereas Escherichia coli is not. Please describe the chemical reactions of the test that you will use to show this.
- 2. What sample shows a positive result with the Molisch Test? Why did this sample give a positive result? 3. What is the chemical basis of the Xanthoproteic Test? 4. Egg white and milk are used as antidotes for heavy metal poisoning. Explain. 5. According to advertisements, commercial shampoo or conditioner contains “protein." Suggest a test that could be done on the product to check this claim? Explain your answer.1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.1. Determine the inactivation rate constant (K) for a microorganism for the following treated effluent sample. The effluent temperature was 20°C. If the activation energy for the disinfection reaction is 60 kJ/mole, determine the inactivation rate constant (K) at 12°C. Assume Chick's law applies. In (N/N.) Time, min 1.5 3.9 3 2.5 5.6 7.7 9.2
- 1. Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:1. Why does a precipitate form on Sierra's medium when incoulated with a lipolytic organism? Give the reasons and explain it thoroughly.Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?