1. What is the principle involved in chromatography? 2. What factors determine the rate of migration of a solute? 3. In a chromatogram, what does it mean if the spot is at the solvent front?
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- 25. In the paper chromatography experiment on food dyes, salt water was used as the A. mobile phase B. stationary phase C. eluent D. solute E. sample spots5. Which term is INCORRECTLY defined? a. linear range - the range over which the response of an analytical method is proportional to analyte concentration b. dynamic range concentration range over which there is a measurable response to analyte, though the response is not linear c. blank solution - a solution containing all reagents and solvents used in the analysis to which no analyte is deliberately added d. standard solutions - solutions for which the standard deviation is known - 6. True or False: Internal standards correct random error caused by run-to-run variation. a. True b. FalseWhich form of chromatography is INCORRECTLY defined? A. partition chromatography – solute equilibrates between the stationary liquid and the mobile phase B. affinity chromatography – solute binds to a molecule specific for the solute C. molecular exclusion chromatography – solute is separated based on size D. adsorption chromatography – solute is adsorbed into solid particles E. ion-exchange chromatography – solute ions of the opposite charge are attracted to the stationary phase
- Which statement about the determination of the percentage purity of benzoic acid is correct? * A- Only sodium hydroxide solution can be used as the titrant in the volumetric analysis of benzoic acid. B- Analysing a benzoic acid sample that still contains some hydrochloric acid may yield an analyte percentage that is less than expected. C- Analysing a wet benzoic acid sample may yield an analyte percentage that is more than expected. D- All these statements are correct. E- None of these statements are correct.3. What is particulate gravimetry? When do we use this technique? 4. What are the conditions for a successful gravimetric analysis. 5. Define nucleation. What conditions are important for this stage.22 Experiment 2 Chromatography 32. A student properly sets up a column chromatography experiment. Although the mixture is soluble in the mobile phase, the sample appears "stuck" at the top of the column and will not elute regardless of how much solvent is applied. What could be the cause of this prob- lem and what could the student do to try and achieve a successful separation? NA disolves in #20 Salt No maybe the compound NASI is to much an the solvent being #20 is to little Vodafo 3. A student performs a separation by TLC on a silica get plate that results in three spots. A red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm away, and a yellow spot is 49 mm away. The distance from the original spot to where the solvent stopped is 55 mm. a) Calculate the Re value for each component in the mixture. (Show your work and report the correct number of significant figures.) highest polarity. Explain your answer. b) The silica gel plate is polar. Rank the three…
- Pls do fast within 5 minutes and i will give like for sure Solution must be in typed form To plan a liquid–liquid extraction we need to know the solute’s distribution ratio between the two phases. One approach is to carry out the extraction on a solution that contains a known amount of solute. After the extraction, we isolate the organic phase and allow it to evaporate, leaving behind the solute. In one such experiment, 1.235 g of a solute with a molar mass of 117.3 g/mol is dissolved in 10.00 mL of water. After extracting with 5.00 mL of toluene, 0.889 g of the solute is recovered in the organic phase. If we extract 20.00 mL of an aqueous solution that contains the solute using 10.00 mL of toluene, what is the extraction efficiency?1. In thin layer chromatography (TLC), why do you need to use a pencil in marking the spots, origin, and solvent front and not a marker? 2. Why is it necessary to mark the solvent front immediately? 3. Why do you need to ensure that the loading spot is above the solvent level in paper chromatography? 4. Why do different compounds travel different distances on the piece of paper?In a thin layer chromatography (TLC) experiment, solute A traveled 20 mm while solute B traveled 30 mm. The solvent traveled 40 mm. Which has the greater affinity for the solvent? a. not enough data b. solute B c. solute A d. both A & B have equal affinity
- 1. Why is it necessary to cover the developing chamber tightly during the development of a chromatogram? 2. Can paper chromatography be used to separate and identify very volatile substances? Explain your answer.Why is recrystallization not applicable to samples which contain large amount of impurities?From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?