1. In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin) dissolved in H2O to a final volume of 5 mL was used as stock solution. What is the weight of BSA in 0.1 mL of stock solution? In 0.2 mL? The mentioned aliquots (#9) which were diluted with enough water to a final volume of 1 mL were assayed colorimetrically and yielded the following absorbance readings: mL stock mL H2O Absorbance 0.2 0.8 0.100 0.4 0.6 0.200 0.6 0.4 0.300 0.8 0.2 0.400 1.0 0.0 0.500 i. Tabulate mg BSA vs. absorbance reading ii. Draw the curve where mg BSA is on x-axis and absorbance on y-axis (use graphing paper). iii. Calculate the slope of the curve iv. If a sample solution has an absorbance of 0.332, determine mg protein of the sample.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
1. In the preparation of the standard curve for protein analysis, 50 mn
BSA (bovine serum albumin) dissolved in H2O to a final volume of 5
mL was used as stock solution. What is the weight of BSA in 0.1 mL of
stock solution? In 0.2 mL? The mentioned aliquots (#9) which were diluted with enough water to a final volume of 1 mL were assayed colorimetrically and yielded the following absorbance readings:
mL stock mL H2O Absorbance
0.2 0.8 0.100
0.4 0.6 0.200
0.6 0.4 0.300
0.8 0.2 0.400
1.0 0.0 0.500
i. Tabulate mg BSA vs. absorbance reading
ii. Draw the curve where mg BSA is on x-axis and absorbance on
y-axis (use graphing paper).
iii. Calculate the slope of the curve
iv. If a sample solution has an absorbance of 0.332, determine
mg protein of the sample.
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