1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. a. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized fusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased, PCR. Since everyone has DNA that is unique, it was an obvious match- b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant dadong XAHA Seized Tusk Allele 1: Allele 2: to yonou Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 FH19 FH60 FH67 FH71 147 97 62 147 103 62 Elephant 10 193 193 FH19 189 193 185 193 183 185 193 193 187 187 187 193 193 193 193 193 193 193 FH60 FH67 FH71 147 97 147 97 147 95 147 147 95 103 147 97 147 147 105 91 145 95 147 95 145 91 147 97 149 97 151 147 97 97 147 97 147 97 189 147 193 147 105 62 64 95 ៩ ៩ ៩៩៩ ៩៩ ៨ ៩ ៩ |8d| ថ ៨ ៩ ៩ ៩ 62 62 62 64 62 64 62 64 64 64 60 147 103 62 62 62 62 62 62 FH129 152 160 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

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Chapter10: Biotechnology
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Please solve question b) and c) * The seized tusks came for elephant #8.
1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime
where 10 elephants from the same population were found dead with their tusks removed. The genotype of the
seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci
correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently
slaughtered elephants.
C.
Did the seized tusk come from one of the recently
slaughtered elephants? If so, which one, or if no,
why not? The seized tusks came from
elephant #8. This is because the seized
elephants matches Perfectly with the decreased
PCR. Since everyone has DNA that is unique,
it was an obvious match-
a.
b. For each of the 5 STR loci, draw the expected
pattern of bands on the gel below. Show the alleles
for the seized tusk and the elephant you identified
as a potential match. Also, include a DNA ladder
that has DNA fragments that are 75, 125, 150, and
175 base pairs (bp) in size.
Base
Pairs
200
180
160
140
120
DNA
Ladder
Seized
Tusk
Matching
Elephant
yillidsdong 201
yonsupett
lugog ome
Seized Tusk FH19 FH60
Allele 1:
Allele 2:
Elephant 1
T
able 1: Genotype for five STR loci recovered from a
seized elephant tusk.
Elephant 2
Elephant 3
Elephant 4
Elephant 5
Elephant 6
Elephant 7
Elephant 8
FH67 FH71
193 147 97 62
193 147 103 62
Elephant 9
Elephant 10
FH19
FH60
FH67 FH71
189
147 97
62
193
147 97
64
185
147 95
62
193
147
62
183
147 95
62
185
147
97
64
193
147
91
62
193
147
105
64
187
145
95
62
187
147 95
64
187
145
91
64
193
147 97
64
193
149 97
60
193
151
97
62
193
147
97
62
193 147
103
62
193 147
97
62
193
147
97
62
189
147
95
62
193 147 105 62
FH129
152
160
103
FH129
152
154
152
152
152
162
152
152
152
160
152
160
156
160
152
160
160
160
152
156
T
Table 2: Genotypes using five STR loci for 10 slaughtered
elephants.
How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized
tusks?
Transcribed Image Text:1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized tusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased PCR. Since everyone has DNA that is unique, it was an obvious match- a. b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant yillidsdong 201 yonsupett lugog ome Seized Tusk FH19 FH60 Allele 1: Allele 2: Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 FH67 FH71 193 147 97 62 193 147 103 62 Elephant 9 Elephant 10 FH19 FH60 FH67 FH71 189 147 97 62 193 147 97 64 185 147 95 62 193 147 62 183 147 95 62 185 147 97 64 193 147 91 62 193 147 105 64 187 145 95 62 187 147 95 64 187 145 91 64 193 147 97 64 193 149 97 60 193 151 97 62 193 147 97 62 193 147 103 62 193 147 97 62 193 147 97 62 189 147 95 62 193 147 105 62 FH129 152 160 103 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?
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