Fruit Fly Lab Report

For one of the monohybrid crosses you performed in this Investigation, describe how to use the phenotype ratios to determine

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

It was decided that there would be 80 vestigial flies and 20 wild type flies to total to an initial population of 100 drosophila. Next, the flies were anesthetized flies using Fly Nap. The flies were counted out to reach desired ratio, sexing the flies making sure there are equal amounts of males and females to be sure there is ample individuals to allow successful mating. The fly’s food was prepared by taking a frozen rotten banana, cutting it in half, mashing up the banana meat, and mixing yeast into it. The

Now you have determined some facts about the grounded allele and the trait that it causes. Given what you know, do you expect the mutant F1 flies to be homozygous or heterozygous for the allele that causes the grounded trait? According to your reasoning, if you mated two mutant F1 flies, what percentage of flies would you expect to be wild type versus mutant in the F2 progeny? Draw a Punnett square of this cross to justify your answer.

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

This determination was mainly so the group could easily spot the sex and traits of the flies for later steps. This was done to all four tubes of the P generation, all of the adult females were placed disposed of roughly 30 males from the wild type were kept in a separate jar. The adult females were not wanted because the females could have already mated with the males and the group needed the control of the transfer of genetics for the test crosses. The new virgins were born and collected approximately eight hour after the removal of the adults and placed into separate jars labeled by the genotype of their parents. After the process was done and 5-6 virgin females were collected the process could continue in order to cross still the P generation.

The Drosophila melanogaster is a fruit fly with a very short life cycle. They can be winged or wingless, and have red eyes or white eyes. The different options are called alleles. Alleles are the variants of a specific gene, and one is received from each parent on each chromosome. (“What Are Dominant and Recessive?”). It was chosen to use winged females and wingless males to predict the offspring in this experiment. The winged allele is dominant, meaning it only needs one allele to physically appear. The wingless allele is recessive, which gets covered up by the dominant allele (“Fruit Fly Genetics”). Each trait has two alleles in the flies’

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

Drosophila melanogaster (fruit fly) was chosen as the organism of study due to its rapid life cycle, taking only 2-3 weeks for new offspring to come to maturity, as well as the ability to easily phenotype individual flies. The procedure for this experiment was performed twice, once for a small population of parental fruit flies, consisting of 2 wild type females (tan pigment), two mutant type females (ebony pigment), two wild type males (tan pigment), and two mutant type males (ebony pigment). The second group consisted of 10 females of each the wild and mutant type flies and 10 males of each the wild and mutant type flies. All parental fruit flies were true breeding.

The main purpose for this conducting this experiment was to further knowledge on Mendelian Genetics and how traits are inherited from generation to generation. Something that we attempted to solve was which traits were considered dominant and which were considered to be recessive. Drosophila melanogaster also known as fruit flies were used in this experiment, Dihybrid crosses were done to gather information on how characteristics are linked from generation to generation. Our crosses consisted of female wild type with male sepia eye/ ebony body and female ebony with male vestigial. It is shown that some inheritance patterns are due to unlinked genes and linked genes.

In the F-1 generation, there is 1:0 ratio of Acid to Lightning, so 100% of the offspring have Acid breath. The shown phenotype is the Dominant (Acid), and the hidden phenotype is Recessive (Lightning). There is a 3:1 ratio in the F-2 (of Acid to Lightning), which means that the inheritance is a simple dominant/recessive. In both the original and reciprocal generations, the same ratios are present, so simple dominant/recessive inheritance applies for both. For the body color, the traits are sex linked, and Red is dominant to Gold. In the original cross F-1 generation, there is a 1:1 ratios of Red to Red between males and females, and there are no Gold bodies. In the F-2 generation, there is a 2:1 ratio of Red to Gold for males, and there are no female Reds, thus it is sex linked. The phenotype of redness is connected to the gender. For the reciprocal cross in the F-1, the females are Red and the males are Gold; there is a 1:1 ratio for Red females to Gold males. The F-2 has a 1:1:1:1 ratio of female Red to male Red to Female Gold to Male Gold. These results further conclude that the inheritance is sexually linked because the father of the reciprocal cross was Red, not

First step, one of the major issues was that all members in the group did not pay attention to the gender of flies in each vial of the unknown P cross of the F1 generation. The number of male and female might not equal to ratio 1:1 in each vial which led to have an error because Bar eye mutation is a dominant X- linked gene that does not assort independently while vestigial wing mutation is autosomal mutation located on chromosome number 2. As a result, vestigial gene assorts independently and does not play significant role with gender in the next generation: however, gender played significant role when determining mutation specifically for Bar eye mutation. Second, there was no differences in the traits as flies observed in first count on April 10 and second count on April 17. Third, there was a difficulty while transferring flies from the vails to the nap chamber. Flies in some vials remained sticking in the food gel due to knocking issue. Some vials accidently knocking aggressively made the flies stick in the food gel. Also, some fruit flies flied and some of them killed accidently while putting the stopper because transferring the flies were quickly from the vials to the nap chamber. Growth condition were not good for some vials that made the group to discard some vials and used the backup vials due to contamination and bacterial infection that killed the flies in some vials. Finally, according to table 4, the individual Chi square for vial A, C, and D was lower than the critical value and support the hypothesis; however, Vial E showed Chi square value greater than the critical value which rejected the hypothesis. Vial E during the second count was not knocked to get the flies away from the stopper which made some of them to fly while transferring them to the nap

The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.

First, a female and a male were selected as parents with two traits (female with red eyes and vestigial wings, male with white eyes and normal wings) at the construction kit tab. They were then bred together and one female and one male is then selected from the first 100 off springs (F1) to be used as parents for F2 off springs. They are stored in the future parents jar. Next, the parent and offspring jars are cleared and the 2nd gen parents were transferred into the parent jar to allow breeding again. This time, the number of off springs for each phenotype (red/white eyes, normal/vestigial wings) and sex group (male/female) are sorted out and