BIO250 Lab 2 ASSIGNMENT

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School

Miami Dade College, Miami *

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Course

250

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Biology

Date

May 10, 2024

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docx

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Student Name: Access Code (located on the lid of your lab kit): Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet. Submissions without proper formatting, all required photos or sufficient responses will be rejected. Pre-lab Questions • This lab includes two experiments that each look at different aspects of culturing microorganisms onto growth media. What concepts does each experiment focus on? Why do you think these concepts are important to the field of microbiology? The concepts each of these experiments focuses on is mainly on the fundamental concepts in which are related to the cultivation, maintenance and manipulation of the microorganisms in the setting of a labratory. Such concepts are crucially essential for research and diagnosis as well as the processes of biotechnology, and medicine. • In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools and provide a scenario in which you would use each tool. • Innoculation Loops : This tool would be used in assisting to move microorganisms from one plate to another. • Sterile Plate Spreaders: This is the technique for spread plate to incoculate plates. • Innoculation Needles: These will be used in order to inoculate semi-solid media or stab tubes. It will gather germs from certain areas of culture. • Sterile Cotton Swabs : these will be used to obtain microbiological samples from a range of surfaces in order to initiate a culture plate. • Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes. Focus particularly on those used with E. coli used to make human insulin. A chemostat is an apparatus utilized to perpetually regulate the growth pattern of laboratory cultures. Constantly supplementing with new medium inhibits the growth of essential nutrients, facilitates bacterial expansion, and eradicates byproducts of bacterial cells. • You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to diagnose a gastrointestinal infection. You streak your sample on a growth media containing glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7 . You incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see clear bacterial colonies forming on the plate. Would you feel confident in stating that you had successfully cultured all the bacteria from your gut sample? Why or why not?

I am certain that the bacteria from the gut sample that I cultivated were present due to the presence of bacterial proliferation. EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION Introduction Questions • Describe the objectives of Experiment 1. State the goal of the experiment and the learning outcome that it is trying to convey to you as a microbiology student. The purpose of this investigation is to provide students with the practical knowledge required for laboratory work. Students will learn the precise procedures for preparing agar plates, including the measurement, combining, and sterilization of nutrient-containing agar. Additionally, the experiment endeavors to instruct students on the correct techniques for inoculating bacteria, such as sprinkling or spreading in order to establish isolated colonies. By inoculating agar plates with an infection, students are able to observe and analyze the growth patterns of bacterial colonies. This enables them to gain insight into the way in which the conditions of incubation influence the progression of microorganisms. • Proper aseptic technique is crucial to ensuring the growth of pure bacterial colonies. What will you do in this lab to demonstrate that you are using proper aseptic technique? One feasible strategy for validating the proper implementation of the aseptic technique is to limit the duration of air exposure for media while it is in an open position. In a laboratory setting, what are three ways you can properly sterilize culturing equipment? • Filter sterilization • Dry heat sterilization • Filter sterilization • What method of sterilization will you use in this experiment? Autoclaving • What results do you expect to see with this experiment? For example, do you expect to see growth from all surfaces or just some? Are there particular strains you expect from the surfaces you swabbed? The expected consequence is the significant proliferation of microorganisms on agar plates after injection. The goal of achieving well-isolated colonies is to make it easier to observe and examine

different morphologies. The inquiry anticipates uncovering a diverse array of microbial flora, which will vary based on the surfaces that are swabbed. Different strains or species will result in distinct appearances of the colonies. It is crucial to exhibit aseptic techniques, and any unexpected proliferation might indicate contamination. The primary aim of the experiment is to provide a deeper understanding of bacterial isolation, growth, and the range of microorganisms found on the collected surfaces, regardless of the individual bacterial strains, which are influenced by environmental conditions. Data and Observations In the table below, report your observed growth corresponding to each plate number and its corresponding source. Then provide a photo of your growth plates. Your data should have a quantitative aspect to it, such as a count of the number of colonies that grew. Your data must also correspond to the photo you include for credit. Table 1: Colony Growth Plate Number Source Growth (Color, Amount, Shape, etc.) 1 Bottom Shoe Amount: 3 small and 2 large colonies Shape: Circular Color: White 2 Mouth Color: White Shape: Circular Amount: 2 small colonies 3 Control No growth 4 table Color: Yellow Shape: Circular Amount: 4 small colonies 5 Airborne control No growth Provide a clear, high resolution photo of your plates after incubation with the lids removed . For credit on this lab, your photo must show the growth that is reported in your tabulated data and must also include your handwritten name in the background.

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